ChromoTek NEDD8-Trap Agarose, Kit for Immunoprecipitation

Validation Data Gallery

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  NEDD8-Trap Agarose (MG-3002A) was used to immunoprecipitate endogenous NEDD8 and NEDDylated proteins from human HEPG2 cells. Lysis was achieved with RIPA buffer. For each IP, samples of the input lysate (I), non-bound flow-through (FT), and bound (B) fractions were analyzed via Coomassie stained SDS-PAGE & Western blot. Anti-NEDD8 polyclonal antibody (16777-1-AP) and Multi-rAb® HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) were used in the Western blot analysis. The Trap shows efficient IP of endogenous NEDD8 and NEDDylated proteins with low background.

• 

  NEDD8-Trap Agarose (MG-3002A) was used to immunoprecipitate endogenous NEDD8 and NEDDylated proteins from human HEPG2 cells. Lysis was achieved with standard Lysis buffer. For each IP, samples of the input lysate (I), non-bound flow-through (FT), and bound (B) fractions were analyzed via Coomassie stained SDS-PAGE & Western blot. Anti-NEDD8 polyclonal antibody (16777-1-AP) and Multi-rAb® HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) were used in the Western blot analysis. The Trap shows efficient IP of endogenous NEDD8 and NEDDylated proteins with low background.

• 

  NEDD8-Trap Agarose (MG-3002A) was used to immunoprecipitate endogenous NEDD8 and NEDDylated proteins from different species / cell lines. Lysis was achieved with standard Lysis buffer. For each IP, samples of the input lysate (I), non-bound flow-through (FT), and bound (B) fractions were analyzed via Western blot. Anti-NEDD8 polyclonal antibody (16777-1-AP) and Multi-rAb® HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) were used in the Western blot analysis. The Trap shows efficient IP of endogenous NEDD8 and NEDDylated proteins with low background.

The ChromoTek NEDD8-Trap Agarose, Kit for Immunoprecipitation consists of an anti-NEDD8 VHH, which is coupled to agarose beads. It also contains lysis, wash, and elution buffers that can be used for the immunoprecipitation of NEDD8 tagged proteins from cell extracts of various organisms.

Cat No. MG-3002AK

Reactivity

NEDD8

Applications

IP, Co-IP

Host/Type

Alpaca, recombinant VHH

Conjugate

Agarose

Synonyms

Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8, Ubiquitin-like protein NEDD8

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Product Information

The ChromoTek NEDD8-Trap Agarose, Kit for Immunoprecipitation consists of an anti-NEDD8 VHH, which is coupled to agarose beads. It also contains lysis, wash, and elution buffers that can be used for the immunoprecipitation of NEDD8 tagged proteins from cell extracts of various organisms.

• Description: The NEDD8-Trap Agarose Kit contains NEDD8-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of NEDD8 proteins.;

  • Fast, reliable & efficient one-step immunoprecipitation;

  • Ready-to-use;

  • Stable in harsh buffer conditions;

  • Suitable for downstream mass spec analysis;

  • High Specificity;

  • Low background

• Applications: IP, Co-IP
• Specificity/Target: /
• Binding Capacity: /
• Bead concentration: 50% Slurry
• Resin Type: Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
• Elution buffer: 2x SDS-sample buffer (Lämmli), 200 mM glycine pH 2.5
• Wash Buffer Compatibility: 2M NaCl, 5 mM DTT, 5 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0.05% SDS, 3-4 M Urea
• Type: VHH
• Class: Recombinant - Animal free production
• Host: Alpaca
• Affinity: 350 nM
• Compatibility with mass spectrometry: The NEDD8-Trap is optimized for on-bead digestion. For the application note, please click here: ; On-bead digest protocol for mass spectrometry
• RRID: AB_3740244
• Storage Buffer: 20% Ethanol
• Storage Condition: Upon arrival store at +4°C / do not freeze!
• Shipping: Ambient Temp

Kit components

• Component: Description
• NEDD8-Trap Agarose: 20 reactions (500 µl)
• Lysis buffer: Optimized for cytoplasmic proteins and mammalian cell lysis
• RIPA buffer: Optimized for nuclear/chromatin proteins and mammalian cell lysis
• RIPA buffer: Optimized for nuclear/chromatin proteins and mammalian cell lysis
• Wash buffer: Removal of unwanted proteins, peptides, etc.
• Dilution buffer: Dilution of cell lysate
• Elution buffer: For acidic elution

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