ChromoTek NEDD8-Trap Agarose

Validation Data Gallery

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  NEDD8-Trap Agarose (MG-3002A) was used to immunoprecipitate endogenous NEDD8 and NEDDylated proteins from human HEPG2 cells. Lysis was achieved with RIPA buffer. For each IP, samples of the input lysate (I), non-bound flow-through (FT), and bound (B) fractions were analyzed via Coomassie stained SDS-PAGE & Western blot. Anti-NEDD8 polyclonal antibody (16777-1-AP) and Multi-rAb® HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) were used in the Western blot analysis. The Trap shows efficient IP of endogenous NEDD8 and NEDDylated proteins with low background.

• 

  NEDD8-Trap Agarose (MG-3002A) was used to immunoprecipitate endogenous NEDD8 and NEDDylated proteins from human HEPG2 cells. Lysis was achieved with standard Lysis buffer. For each IP, samples of the input lysate (I), non-bound flow-through (FT), and bound (B) fractions were analyzed via Coomassie stained SDS-PAGE & Western blot. Anti-NEDD8 polyclonal antibody (16777-1-AP) and Multi-rAb® HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) were used in the Western blot analysis. The Trap shows efficient IP of endogenous NEDD8 and NEDDylated proteins with low background.

• 

  NEDD8-Trap Agarose (MG-3002A) was used to immunoprecipitate endogenous NEDD8 and NEDDylated proteins from different species / cell lines. Lysis was achieved with standard Lysis buffer. For each IP, samples of the input lysate (I), non-bound flow-through (FT), and bound (B) fractions were analyzed via Western blot. Anti-NEDD8 polyclonal antibody (16777-1-AP) and Multi-rAb® HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) were used in the Western blot analysis. The Trap shows efficient IP of endogenous NEDD8 and NEDDylated proteins with low background.

The ChromoTek NEDD8-Trap Agarose consists of an anti-NEDD8 VHH (Nanobody), which is coupled to agarose beads. It can be used for the immunoprecipitation of NEDD8 tagged proteins from cell extracts of various organisms.

Cat No. MG-3002A

Reactivity

NEDD8

Applications

IP, Co-IP

Host/Type

Alpaca, recombinant VHH

Conjugate

Agarose

Synonyms

Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8, Ubiquitin-like protein NEDD8

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Product Information

The ChromoTek NEDD8-Trap Agarose consists of an anti-NEDD8 VHH (Nanobody), which is coupled to agarose beads. It can be used for the immunoprecipitation of NEDD8 tagged proteins from cell extracts of various organisms.

• Description: Ready to use VHH coupled Agarose Beads for Immunoprecipitation of NEDD8.;

  • Fast, reliable & efficient one-step immunoprecipitation;

  • Ready-to-use;

  • Stable in harsh buffer conditions;

  • Suitable for downstream mass spec analysis;

  • High Specificity;

  • Low background

• Applications: IP, Co-IP
• Specificity/Target: /
• Binding Capacity: /
• Bead concentration: 50% Slurry
• Resin Type: Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
• Elution buffer: 2x SDS-sample buffer (Lämmli), 200 mM glycine pH 2.5
• Wash Buffer Compatibility: 2M NaCl, 5 mM DTT, 5 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0.05% SDS, 3-4 M Urea
• Type: VHH
• Class: Recombinant - Animal free production
• Host: Alpaca
• Affinity: 350 nM
• Compatibility with mass spectrometry: The NEDD8-Trap is optimized for on-bead digestion. For the application note, please click here: ; On-bead digest protocol for mass spectrometry
• RRID: AB_3740244
• Storage Buffer: 20% Ethanol
• Storage Condition: Upon arrival store at +4°C / do not freeze!
• Shipping: Ambient Temp

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