ChromoTek RFP-Trap® Magnetic Particles M-270 Kit

RFP-Trap® Magnetic Particles M-270 for immunoprecipitation (IP) of RFP-tagged proteins. RFP-Trap Magnetic Particles M-270 is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a RFP VHH/ Nanobody coupled to Magnetic Particles M-270.

Specificity

mCherry, mRFP, mPlum

Applications

IP, CoIP

Conjugate

Magnetic Particles M-270

Cat no : rtdk

Synonyms

RFP, RFP tag, RFP-tag, RFP-Trap, RFP-Trap® Magnetic Particles M-270, RFP-Trap® Magnetic Particles M-270 Kit, rtdk, rtdk-20



Product Information

RFP-Trap® Magnetic Particles M-270 for immunoprecipitation (IP) of RFP-tagged proteins. RFP-Trap Magnetic Particles M-270 is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a RFP VHH/ Nanobody coupled to Magnetic Particles M-270.

ApplicationsIP, CoIP
Clonality VHH
HostAlpaca
AffinityDissociation constant KD of 5 nM
Specificity/TargetmRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet
Coupled VHHRecombinant, monoclonal anti-Red Fluorescent Protein (RFP) single domain antibody (sdAb) fragment
ConjugateMagnetic Particles M-270
Beads~ 2.8 µm (Magnetic, high throughput-compatible)
Binding capacity10 µL slurry bind ~ 0.5 µg of recombinant mCherry
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Storage Buffer1x PBS, Preservative: 0.09 % sodium azide
Storage Condition Upon receipt store at +4°C. Do not freeze!

Kit components

ComponentDescription
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution
RFP-Trap Magnetic Particles M-27020 reactions (500 µL)