CoraLite®555-conjugated SLP76 Monoclonal antibody

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  IF Staining of HeLa using CL555-66465

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®555 SLP76 antibody (CL555-66465, Clone: 4H10B1 ) at dilution of 1:200.

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  FC experiment of Jurkat using CL555-66465

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human SLP76 (CL555-66465, Clone:4H10B1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

SLP76 Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL555-66465

Clone No.4H10B1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

LCP2, 4H10B1, Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76 kDa, SLP 76

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66465 targets SLP76 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3432

  Product name: Recombinant human LCP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 159-533 aa of BC016618

  Sequence: SILPAKPFPNSNSMYIDRPPSGKTPQQPPVPPQRPMAALPPPPAGRNHSPLPPPQTNHEEPSRSRNHKTAKLPAPSIDRSTKPPLDRSLAPFDREPFTLGKKPPFSDKPSIPAGRSLGEHLPKIQKPPLPPTTERHERSSPLPGKKPPVPKHGWGPDRRENDEDDVHQRPLPQPALLPMSSNTFPSRSTKPSPMNPLPSSHMPGAFSESNSSFPQSASLPPYFSQGPSNRPPIRAEGRNFPLPLPNKPRPPSPAEEENSLNEEWYVSYITRPEAEAALRKINQDGTFLVRDSSKKTTTNPYVLMVLYKDKVYNIQIRYQKESQVYLLGTGLRGKEDFLSVSDIIDYFRKMPLLLIDGKNRGSRYQCTLTHAAGYP

• Full Name: lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa)
• Calculated Molecular Weight: 533 aa, 60 kDa
• GenBank Accession Number: BC016618
• Gene Symbol: SLP76
• Gene ID (NCBI): 3937
• RRID: AB_2919685
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q13094
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

LCP2, also named as SLP76, is an adapter protein that acts as a substrate of the T cell antigen receptor (TCR)-activated protein tyrosine kinase pathway. It contains an N-terminal leucine Zip motif, a tyrosine-rich domain, a proline-rich domain and a C-terminal SH2 domain. It is constitutively associated with the growth factor receptor-bound protein 2 (Grb2)-related adapter protein 2 (Grap2, a.k.a. GADS) through the interaction of its proline residues with one of the SH3 domains of Grap2. SLP76 associates with growth factor receptor bound protein 2 (Grap2), and is thought to play a role TCR-mediated intracellular signal transduction. SLP76 is also expressed by mast cells, and mast cell activation was markedly impaired in SLP76-deficient mice.