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CoraLite®594-conjugated SLP76 Monoclonal antibody

SLP76 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4H10B1

Cat no : CL594-66465

Synonyms

LCP2, Lymphocyte cytosolic protein 2, SLP 76, SLP 76 tyrosine phosphoprotein, SLP76

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated SLP76 Monoclonal antibody (CL594-66465)

    IF Staining of HeLa using CL594-66465

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-66465 (SLP76 antibody) at dilution of 1:100.

  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite®594-conjugated SLP76 Monoclonal antibody (CL594-66465)

    FC experiment of Jurkat using CL594-66465

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human SLP76 (CL594-66465, Clone:4H10B1) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66465 targets SLP76 in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SLP76 fusion protein Ag3432
Full Name lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa)
Calculated Molecular Weight 533 aa, 60 kDa
GenBank Accession NumberBC016618
Gene Symbol LCP2
Gene ID (NCBI) 3937
RRIDAB_2883584
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LCP2, also named as SLP76, is an adapter protein that acts as a substrate of the T cell antigen receptor (TCR)-activated protein tyrosine kinase pathway. It contains an N-terminal leucine Zip motif, a tyrosine-rich domain, a proline-rich domain and a C-terminal SH2 domain. It is constitutively associated with the growth factor receptor-bound protein 2 (Grb2)-related adapter protein 2 (Grap2, a.k.a. GADS) through the interaction of its proline residues with one of the SH3 domains of Grap2. SLP76 associates with growth factor receptor bound protein 2 (Grap2), and is thought to play a role TCR-mediated intracellular signal transduction. SLP76 is also expressed by mast cells, and mast cell activation was markedly impaired in SLP76-deficient mice.

Protocols

Product Specific Protocols
IF protocol for CL594 SLP76 antibody CL594-66465Download protocol
FC protocol for CL594 SLP76 antibody CL594-66465Download protocol
Standard Protocols
Click here to view our Standard Protocols