CoraLite®594-conjugated SPAG5 Polyclonal antibody

• Featured Product
• KD/KO Validated

SPAG5 Polyclonal Antibody for IF, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-14726

Synonyms

Astrin, DEEPEST, hMAP126, MAP126, SPAG5, sperm associated antigen 5

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Validation Data Gallery

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  IF Staining of HeLa using CL594-14726

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 SPAG5 antibody (CL594-14726) at dilution of 1:200.

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  FC experiment of HeLa using CL594-14726

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human SPAG5 (CL594-14726) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-14726 targets SPAG5 in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPAG5 fusion protein Ag6485
• Full Name: sperm associated antigen 5
• Calculated Molecular Weight: 134 kDa
• Observed Molecular Weight: 135 kDa, 150 kDa
• GenBank Accession Number: BC000322
• Gene Symbol: SPAG5
• Gene ID (NCBI): 10615
• RRID: AB_2919813
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPAG5, also named as MAP126, Astrin and Deepest, is necessary for normal spindle formation during mitosis. It is highly expressed in testis. SPAG5 has different localization in somatic versus male germ cells suggesting the possibility of different function. This antibody is specific to SPAG5.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C