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CoraLite® Plus 488-conjugated ValRS Monoclonal antibody

ValRS Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A2C3

Cat no : CL488-67935

Synonyms

G7A, Protein G7a, Valine tRNA ligase, ValRS, valyl tRNA synthetase, VARS, VARS1, VARS2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated ValRS Monoclonal ant (CL488-67935)

    IF Staining of HeLa using CL488-67935

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 ValRS antibody (CL488-67935, Clone: 1A2C3 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated ValRS Monoclonal ant (CL488-67935)

    FC experiment of HeLa using CL488-67935

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ValRS (CL488-67935, Clone:1A2C3) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67935 targets ValRS in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ValRS fusion protein Ag9312
Full Name valyl-tRNA synthetase
Calculated Molecular Weight 1264 aa, 140 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC012808
Gene Symbol VARS
Gene ID (NCBI) 7407
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ValRS antibody CL488-67935Download protocol
FC protocol for CL Plus 488 ValRS antibody CL488-67935Download protocol
Standard Protocols
Click here to view our Standard Protocols