|Positive WB detected in||NCCIT cells, HepG2 cells, EC109 cells, K-562 cells, HSCT6 cells, NIH3T3 cells|
|Positive IHC detected in||human breast cancer tissue, human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MDCK cells, HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66320-1-Ig targets Gamma Tubulin in WB, IHC, IF, ELISA applications and shows reactivity with human, dog, mouse, rat samples.
|Tested Reactivity||human, dog, mouse, rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Gamma Tubulin fusion protein Ag23913|
|Full Name||tubulin, gamma 1|
|Calculated molecular weight||51 kDa|
|Observed molecular weight||48-55 kDa|
|GenBank accession number||BC000619|
|Gene ID (NCBI)||7283|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.
Prohibitin, relocated to the front ends, can control the migration directionality of colorectal cancer cells.
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