CoraLite® Plus Fluorescent Dyes

PEGylation is the process of covalently attaching polyethylene glycol (PEG) chains to various molecules like fluorophores to increase their hydrodynamic volume, water solubility and stability. dPEG® (discrete PEG) are molecules with a defined number of ethylene oxide units, thus giving them a specified chain length, molecular weight, and purity compared to traditional PEG with varying sizes and molecular weights. Varying sizes of PEG molecules leads to steric hindrance and other problems like binding/interaction site issues, which result in significant losses of intended activity.

Bioconjugation methods that use aliphatic or hydrophobic linkers for coupling biomolecules often cause problems in the final product due to nonspecific interactions between linker arms. SuperHydrophilic dPEG® technology can significantly improve final conjugate quality by improving specific interactions

Conventional Dye Conjugation

• Lower Degree of Labeling (DOL)
• Higher DOL results in dye-dye quenching
• Conjugates are more likely to aggregate or precipitate during labeling or storage
• Conjugates have more nonspecific binding
• Higher background

Antibody Conjugation with CoraLite® Plus

• High Degree of Labeling (DOL)
• Low levels of dye-dye quenching
• No aggregation or precipitation of labeled molecules
• Conjugates have less nonspecific binding
• Greater signal-to-background ratio

• The spectral properties of Atlantic Blue are similar to Pacific Blue™.
• The spectral properties of Cardinal Red are similar to Texas Red.