|Positive WB detected in||Jurkat cells|
|Positive IHC detected in||human tonsillitis tissue, human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Jurkat cells, human tonsillitis tissue|
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:2000-1:20000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:10000|
|Immunofluorescence (IF)||IF : 1:250-1:1000|
|Sample-dependent, check data in validation data gallery|
60181-1-Ig targets CD3 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Mouse / IgG2b|
|Immunogen||CD3 fusion protein Ag11797|
|Full Name||CD3e molecule, epsilon (CD3-TCR complex)|
|Calculated molecular weight||207 aa, 23 kDa|
|Observed molecular weight||23 kDa|
|GenBank accession number||BC049847|
|Gene ID (NCBI)||916|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a monoclonal antibody directed against the epsilon chain of human CD3 molecule.
|Product Specific Protocols|
|WB protocol for CD3 antibody 60181-1-Ig||Download protocol|
|IHC protocol for CD3 antibody 60181-1-Ig||Download protocol|
|IF protocol for CD3 antibody 60181-1-Ig||Download protocol|
|FC protocol for CD3 antibody 60181-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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