CD3 Antibody

CD3 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

3F3A1

Cat no : 60181-1-Ig

Synonyms

CD3, CD3 epsilon, CD3E, CD3-epsilon, T3E, TCRE



Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inJurkat cells, human tonsillitis tissue
Positive FC detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:1000-1:10000
Immunofluorescence (IF)IF : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 2 publications below

Product Information

60181-1-Ig targets CD3 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD3 fusion protein Ag11797
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Calculated molecular weight 207aa,23 kDa
Observed molecular weight 23 kDa
GenBank accession number BC049847
Gene symbol CD3E
Gene ID (NCBI) 916
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a monoclonal antibody directed against the epsilon chain of human CD3 molecule.

Protocols

Product Specific Protocols
WB protocol for CD3 antibody 60181-1-IgDownload protocol
IHC protocol for CD3 antibody 60181-1-IgDownload protocol
IF protocol for CD3 antibody 60181-1-IgDownload protocol
FC protocol for CD3 antibody 60181-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Immunother Cancer

A PD-L2-based immune marker signature helps to predict survival in resected pancreatic ductal adenocarcinoma.

Authors - Yiyin Zhang
humanIHC

Lupus

Intrarenal macrophage infiltration induced by T cells is associated with podocyte injury in lupus nephritis patients.

Authors - R Ma