GAPDH Antibody 782 Publications

Mouse Monoclonal| Catalog number: 60004-1-Ig |CloneNo.: 1E6D9

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Reviews(2)


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Freight/Packing

Con: 150 μg/150 μl

Choose size:

Please visit your regions distributor:


Species specificity:
human, mouse, rat, zebrafish, yeast, plant, beagle, cow, Cyprinus carpio, Eelworm, fish, hamster, monkey, moth, pig, Porcine Müller, swine, Xenopus laevis

Positive WB detected in:
HeLa cells, arabidopsis whole plant tissue, Raji cells, HEK-293 cells, HepG2 cells, soybean whole plant tissue, whole Nematode tissue, zebrafish tissue, whole yeast cells

Positive IP detected in:
HeLa Cells

Positive IHC detected in:
human kidney tissue, human breast cancer tissue

Positive IF detected in:
Ethacrynic acid treated HeLa cells

Positive FC detected in:
HeLa cells

Recommended dilution:
WB : 1:5000-1:50000
IP : 0.5-4.0 ug for IP and 1:2000-1:12000 for WB
IHC : 1:50-1:500
IF : 1:20-1:200

Product Information


Source:
Mouse

Purification method:
Protein A purification

Isotype:
IgG2b

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
glyceraldehyde-3-phosphate dehydrogenase

Calculated molecular weight:
36 kDa

Observed molecular weight:
36 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
GAPDH

Synonyms
G3PD, GAPD, GAPDH, OK/SW cl.12
Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation.. This antibody is a mouse monoclonal antibody raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

GAPDH Mouse Monoclonal antibody

Qunfeng Wu, 01-11-2018

Applications
Western Blot,
Lot Number
10004129

Primary Antibody Dilution
1:10000
Secondary Antibody Dilution
1:10000

Cell/Tissue Type
Huh7 cells

GAPDH Mouse Monoclonal antibody

Sinu John, 01-10-2018

Applications
Western Blot,
Lot Number
1

Primary Antibody Dilution
1:1000
Secondary Antibody Dilution
1:5000

Cell/Tissue Type
Human THP1


Worked well in the western blot

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