PTGER4 Polyclonal antibody

PTGER4 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 24895-1-AP

Synonyms

EP4, EP4R, PGE receptor EP4 subtype, PGE2 receptor EP4 subtype, Prostanoid EP4 receptor, PTGER2, PTGER4



Tested Applications

Positive WB detected inrat brain tissue, HL-60 cells, mouse brain tissue
Positive IHC detected inhuman prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24895-1-AP is PTGER4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTGER4 fusion protein Ag19217
Full Name prostaglandin E receptor 4 (subtype EP4)
Calculated molecular weight 488 aa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC101534
Gene symbol PTGER4
Gene ID (NCBI) 5734
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PTGER4 antibody 24895-1-APDownload protocol
IHC protocol for PTGER4 antibody 24895-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

J Cell Mol Med

Inhibition of prostaglandin E2 receptor 4 by lnc000908 to promote the endothelial-mesenchymal transition participation in cardiac remodelling.

Authors - Xingxing Chen
mouseWB

JCI Insight

Endothelial cell prostaglandin E2 receptor EP4 is essential for blood pressure homeostasis.

Authors - Hu Xu
IF

Exp Cell Res

Prostaglandin E2 accelerated recovery of chemotherapy-induced intestinal damage by increasing expression of cyclin D.

Authors - Mengzhen Yue
humanWB

Int J Mol Sci

Correlative Study on Impaired Prostaglandin E2 Regulation in Epicardial Adipose Tissue and its Role in Maladaptive Cardiac Remodeling via EPAC2 and ST2 Signaling in Overweight Cardiovascular Disease Subjects.

Authors - Elena Vianello
ratWB

Mol Pharm

PMID: 30109938

Authors - Huan Li
humanWB

J Clin Invest

Antibiotic pretreatment alleviates liver transplant damage in mice and humans.

Authors - Kojiro Nakamura