Anticorps Monoclonal anti-DDDDK tag

DDDDK tag Monoclonal Antibody for ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Protéine recombinante et plus (1)

Applications

WB, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(91)

CloneNo.

1E7B4

Avis (2)

N° de cat : 66008-2-Ig

Synonymes

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Applications publiées

• WB: See 47 publications below
• IF: See 4 publications below
• IP: See 17 publications below
• CoIP: See 5 publications below

Informations sur le produit

66008-2-Ig cible DDDDK tag dans les applications de WB, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Protéine recombinante

• Réactivité: Protéine recombinante
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: DDDDK tag
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DDDDK tag, also known as DDK tag, is a hydrophilic tag for recombinant protein technology with the sequence DYKDDDDK. The structure of DDDDK tag has been optimized for compatibility with the proteins it is attached to and unlikely to denature or inactivated them. For its high hydrophilic nature, theDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag more likely to be accessible to antibodies. This product is generated against 1xFlag tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, no matter it is/they are located at N-terminal, C-terminal or at internal regions. The binding of this product with DDDDK tagged fusion protein does not depend on calcium ion. The clone of this monoclonal antibody hybridoma is 1E7B4, which is different from any other vendors. This DDDDK-tag antibody can be used to detect the DDDDK tagged protein with pCMV-DDDDK, p3XFLAG-CMV, pcDNA3.1-DDDDK, pef-NEO-DDDDK, DDDDK-RIG-I, pLenti6-3XDDDDK and some other vectors.

Publications

Species	Application	Title
	WB,IP	Circ Res; Unspliced XBP1 Confers VSMC Homeostasis and Prevents Aortic Aneurysm Formation via FoxO4 Interaction.; Authors - Guizhen Zhao
	CoIP,WB	Nat Commun; Functional regulation of an ancestral RAG transposon ProtoRAG by a trans-acting factor YY1 in lancelet.; Authors - Song Liu
	WB	Nat Commun; Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.; Authors - Hu Lei
	WB	Bone Res; TAZ inhibits osteoclastogenesis by attenuating TAK1/NF-κB signaling.; Authors - Wanlei Yang
		Nucleic Acids Res; The RNA binding protein EWS is broadly involved in the regulation of pri-miRNA processing in mammalian cells.; Authors - Huiwu Ouyang
		Autophagy; Restoration of CTSD (cathepsin D) and lysosomal function in stroke is neuroprotective.; Authors - M Iqbal Hossain

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yijun (Verified Customer) (01-01-2020)

This tag antibody is with low specificity. Even the samples is not transfected at all (Empty), you will get a band.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293FT, PC3, LNCaP

DDDDK tag Antibody Western Blot, validation (1:1000 dilution) in HEK293FT, PC3, LNCaP (Cat no:66008-2-Ig)

FH

David (Verified Customer) (08-07-2019)

Protein in bacterial expression system with Flag-tag. We ran a chemi blot using anti mouse secondary.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Bacteria

DDDDK tag Antibody Western Blot, validation (1/1000 dilution) in Bacteria (Cat no:66008-2-Ig)

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