Notable Publications - July Update

A small selection of recent notable publications from around the world

What matters to Proteintech is the success of its antibodies in the hands of scientists.

Here’s just a small selection of publications featuring Proteintech products from June - July 2017.

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1. Cell - Ciliary Hedgehog Signaling Restricts Injury-Induced Adipogenesis 2. Nature Medicine - A tripartite complex of suPAR, APOL1 risk variants and αvβ3 integrin on podocytes mediates chronic kidney disease. 3. Molecular Cell - Heterogeneous Ribosomes Preferentially Translate Distinct Subpools of mRNAs Genome-wide. 4. Neuron - A Central Catecholaminergic Circuit Controls Blood Glucose Levels during Stress. 5. Nature Communications - Caffeine inhibits hypothalamic A1R to excite oxytocin neuron and ameliorate dietary obesity in mice.

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1.  Ciliary Hedgehog Signaling Restricts Injury-Induced Adipogenesis

Kopinke, Daniel et al.

Cell, Volume 170, Issue 2, p340–351.e12, 13 July 2017

Highlights:

• Fibro/adipogenic progenitors (FAPs) are dynamically ciliated during regeneration
• Ciliary Hedgehog signaling restricts FAP conversion into adipocytes through TIMP3
• TIMP3 represses injury-induced adipogenesis through inhibition of MMP14
• Removal of cilia from FAPs enhances myofiber size and formation following injury

Cited products: ARL13B & FABP4 antibodies

ARL13B Antibody
KD/KO Validated	Applications: ELISA, IF, IHC, IP, WB
Catalog number: 17711-1-AP	135 Publications
Immunofluorescent analysis of (4% PFA ) fixed MDCK cells using 17711-1-AP( ARL13B Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit

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2. A tripartite complex of suPAR, APOL1 risk variants and αvβ3 integrin on podocytes mediates chronic kidney disease.

Hayek, Salim et al.

Nature  Medicine, doi:10.1038/nm.4362,  26 June 2017

Cited products:  APOL1 ELISA kit

APOL1 ELISA kit
Catalog number:  KE00047	Sensitivity: 0.07 ng/mL
Range: 0.156 ng/mL-10 ng/mL	Tests: 1 X 96 well plate

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3.  Heterogeneous Ribosomes Preferentially Translate Distinct Subpools of mRNAs Genome-wide.

Shi, Zhen et al.

Molecular Cell, 67, 71–83,  July 6, 2017

Highlights:

• Translating ribosomes are heterogeneous at the level of core ribosomal proteins
• Heterogeneous ribosomes preferentially translate distinct subpools of mRNAs
• RPL10A-Ribo-seq enriched mRNAs require RPL10A for their efficient translation 
• IRES elements contribute to the unique translational regulation by RPL10A

Cited products:  EIF3D antibody

EIF3D Antibody
8 Publications	Applications: ELISA, WB, IP, IHC, IF
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10219-1-AP( EIF3D Antibody) at dilution of 1:50 (under 40x lens)	IF result of 10219-1-AP(anti-EIF3D) in U2OS cell (treated with 100 mM sodium arsenite to cause stress-induced translational arrest) by Dr. Nancy Kedersha. U2OS cells; stained with PTG anti-eIF3p66 in green; and counterstained with anti-eIF3b (goat polyclonal) in red; nuclei stained blue using Hoechst.

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4.  A Central Catecholaminergic Circuit Controls Blood Glucose Levels during Stress.

Zhao, Zhe et al.  

Neuron,  Volume 95, Issue 1, p138–152.e5, 5 July 2017

Cited products:  GFP tag antibody

GFP Tag Antibody
Catalog number: 50430-2-AP	63 Pubications
Applications: ELISA, IF, IHC, IP, WB	Rabbit Polyclonal
IP Result of anti-GFP tag (IP:50430-2-AP, 4ug; Detection:50430-2-AP 1:5000) with Transfected HEK-293 cells lysate 300ug.

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5. Caffeine inhibits hypothalamic A1R to excite oxytocin neuron and ameliorate dietary obesity in mice.

Wu, Liufeng et al.

Nature Communications, 8, doi:10.1038/ncomms1590427, June 2017  

Cited products:  MYC-tag

MYC-Tag Antibody
Catalog number: 66004-1-Ig	16 Publications
Applications: ELISA, Co-IP, IF, IP, WB	Mouse Monoclonal
Immunofluorescent analysis of Transfected HEK-293 cells using 66004-1-Ig( MYC-tag Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)	IP Result of anti-MYC-tag (IP:66004-1-Ig, 3ug; Detection:66004-1-Ig 1:5000) with Transfected HEK-293 cells lysate 500ug.

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