Tested Applications
| Positive IF-P detected in | rat heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL405-80008 targets Alpha smooth muscle actin specific in IF-P applications and shows reactivity with human, mouse, rat, pig, chicken, zebrafish samples.
| Tested Reactivity | human, mouse, rat, pig, chicken, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | actin, alpha 2, smooth muscle, aorta |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | NM_001613 |
| Gene Symbol | Alpha smooth muscle actin |
| Gene ID (NCBI) | 59 |
| RRID | AB_3747128 |
| Conjugate | CoraLite® Plus 405 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 399 nm / 422 nm |
| Excitation Laser | Violet Laser (405 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P62736 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 405 Alpha smooth muscle actin specific antibody CL405-80008 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
ANA (Verified Customer) (06-22-2026) | We tested this antibody by flow cytometry on lymph node samples to detect αSMA-positive stromal/fibroblast-like cells and distinguish them from LYVE1-positive endothelial populations. The antibody gave some detectable signal, but only few αSMA-positive fibroblast-like cells were observed. It is unclear whether this reflects a low abundance of αSMA-expressing fibroblasts in lymph nodes or suboptimal staining/antibody performance in our samples. Therefore, it may be useful for αSMA detection, but should be combined with markers such as PDPN/gp38, CD31 and LYVE1.
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