CoraLite® Plus 647-conjugated smooth muscle actin specific Recombinant antibody

smooth muscle actin specific Recombinant Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human, rat, chicken, pig, zebrafish

Applications

IF

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

5H7

Cat no : CL647-80008

Synonyms

a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA

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Validation Data Gallery

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  IF Staining of mouse small intestine using CL647-80008

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-80008, Clone: 5H7 ) at dilution of 1:200, ZG16 antibody (67389-1-Ig, Clone: 1A7B9, red), ASCT2 antibody (20350-1-AP, green).

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  IF Staining of mouse small intestine using CL647-80008

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-80008, Clone: 5H7 ) at dilution of 1:200, ZG16 antibody (67389-1-Ig, Clone: 1A7B9, red), ASCT2 antibody (20350-1-AP, green).

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Tested Applications

• Positive IF detected in: mouse small intestine tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-80008 targets smooth muscle actin specific in IF applications and shows reactivity with mouse, human, rat, chicken, pig, zebrafish samples.

• Tested Reactivity: mouse, human, rat, chicken, pig, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: actin, alpha 2, smooth muscle, aorta
• Calculated Molecular Weight: 42 kDa
• GenBank Accession Number: NM_001613
• Gene Symbol: ACTA2
• Gene ID (NCBI): 59
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.