CoraLite®594-conjugated smooth muscle actin specific Recombinant antibody

smooth muscle actin specific Recombinant Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat, chicken, pig

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5H7

Cat no : CL594-80008

Synonyms

a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA

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Validation Data Gallery

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  IF Staining of mouse heart using CL594-80008

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 smooth muscle actin specific antibody (CL594-80008, Clone: 5H7 ) at dilution of 1:200.

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  FC experiment of C2C12 using CL594-80008

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human smooth muscle actin specific (CL594-80008, Clone:5H7) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: mouse heart tissue
• Positive FC detected in: C2C12 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-80008 targets smooth muscle actin specific in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat, chicken, pig samples.

• Tested Reactivity: Human, mouse, rat, chicken, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: actin, alpha 2, smooth muscle, aorta
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: NM_001613
• Gene Symbol: ACTA2
• Gene ID (NCBI): 59
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.