CoraLite® Plus 488-conjugated CALD1 Monoclonal antibody

• Featured Product
• KD/KO Validated

CALD1 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E1C11

Cat no : CL488-66693

Synonyms

CAD, CALD1, Caldesmon, caldesmon 1, CDM, H CAD, L CAD, NAG22

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-66693

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66693 (CALD1 antibody) at dilution of 1:100.

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  FC experiment of HeLa using CL488-66693

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human CALD1 (CL488-66693, Clone:1E1C11) (green), and 0.2 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Tested Applications

• Positive IF detected in: HepG2 cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

CL488-66693 targets CALD1 in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CALD1 fusion protein Ag13676
• Full Name: caldesmon 1
• Calculated molecular weight: 538 aa, 63 kDa
• Observed molecular weight: 70 kDa~75 kDa
• GenBank accession number: BC040354
• Gene symbol: CALD1
• Gene ID (NCBI): 800
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.