Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 CALD1 antibody (CL555-66693, Clone: 1E1C11 ) at dilution of 1:200.
FC experiment of HeLa using CL555-66693
1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®555 Anti-Human CALD1 (CL555-66693, Clone:1E1C11) (red), or 0.8 ug CoraLite® Plus 555 Mouse IgG2b Isotype Control (MPC-11) (CL555-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®555 Anti-Human CALD1 (CL555-66693, Clone:1E1C11) (red), or 0.8 ug CoraLite® Plus 555 Mouse IgG2b Isotype Control (MPC-11) (CL555-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 CALD1 antibody (CL555-66693, Clone: 1E1C11 ) at dilution of 1:200.
FC (INTRA) Figures
FC experiment of HeLa using CL555-66693
1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®555 Anti-Human CALD1 (CL555-66693, Clone:1E1C11) (red), or 0.8 ug CoraLite® Plus 555 Mouse IgG2b Isotype Control (MPC-11) (CL555-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
CALD1 Monoclonal antibody
Catalog Number
CL555-66693
Citations
-
Dilutions
IF/ICC : 1:50-1:500 FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Applications
IF/ICC, FC (Intra)
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
