CoraLite®555-conjugated CALD1 Monoclonal antibody

• Featured Product
• KD/KO Validated

CALD1 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

1E1C11

Cat no : CL555-66693

Synonyms

CAD, CALD1, Caldesmon, caldesmon 1, CDM, H CAD, L CAD, NAG22

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Validation Data Gallery

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  IF Staining of HeLa using CL555-66693

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 CALD1 antibody (CL555-66693, Clone: 1E1C11 ) at dilution of 1:200.

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  FC experiment of NIH/3T3 using CL555-66693

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human CALD1 (CL555-66693, Clone:1E1C11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: HeLa cells
• Positive FC detected in: NIH/3T3 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

CL555-66693 targets CALD1 in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CALD1 fusion protein Ag13676
• Full Name: caldesmon 1
• Calculated molecular weight: 538 aa, 63 kDa
• Observed molecular weight: 70 kDa~75 kDa
• GenBank accession number: BC040354
• Gene symbol: CALD1
• Gene ID (NCBI): 800
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C