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CALD1 Monoclonal antibody

CALD1 Monoclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

CloneNo.

1E1C11

Cat no : 66693-1-Ig

Synonyms

CAD, CALD1, Caldesmon, caldesmon 1, CDM, H CAD, L CAD, NAG22



Tested Applications

Positive WB detected inNCI-H1299 cells, HeLa cells, HepG2 cells, PC-3 cells, SKOV-3 cells, A2780 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman hysteromyoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66693-1-Ig targets CALD1 in WB, IHC, IF, FC,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CALD1 fusion protein Ag13676
Full Name caldesmon 1
Calculated molecular weight 538 aa, 63 kDa
Observed molecular weight 70 kDa~75 kDa
GenBank accession numberBC040354
Gene symbol CALD1
Gene ID (NCBI) 800
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CALD1 antibody 66693-1-IgDownload protocol
IHC protocol for CALD1 antibody 66693-1-IgDownload protocol
IF protocol for CALD1 antibody 66693-1-IgDownload protocol
FC protocol for CALD1 antibody 66693-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols