Anti-Human CD3 (UCHT1) Mouse IgG2a Recombinant Antibody

• 

  FC experiment of human PBMCs using 65570-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD3 (UCHT1) Mouse IgG2a RecAb (65570-1-MR, Clone: UCHT1) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed.

• 

  FC experiment of human PBMCs using 65570-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD3 (UCHT1) Mouse IgG2a RecAb (65570-1-MR, Clone: UCHT1) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed.

CD3 Uni-rAb^® Recombinant Antibody for FC

Cat No. 65570-1-MR

Clone No.UCHT1

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

CD3E, CD3 epsilon, CD3-epsilon, T3E, T-cell surface antigen T3/Leu-4 epsilon chain

---

Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65570-1-MR targets CD3 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor

• Full Name: CD3e molecule, epsilon (CD3-TCR complex)
• Calculated Molecular Weight: 207 aa, 23 kDa
• GenBank Accession Number: BC049847
• Gene Symbol: CD3
• Gene ID (NCBI): 916
• ENSEMBL Gene ID: ENSG00000198851
• RRID: AB_3670324
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P07766
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).