PerCP-Cyanine5.5 Anti-Human CD3 (UCHT1) Mouse IgG2a Recombinant Antibody

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  FC experiment of human PBMCs using CPY5-65570

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD19, and 5 ul PerCP-Cyanine5.5 Anti-Human CD3 (UCHT1) Mouse IgG2a Recombinant Antibody (CPY5-65570, Clone:UCHT1) or unstained. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CPY5-65570

  1x10^6 human PBMCs were surface stained with 5 ul PerCP-Cyanine5.5 Anti-Human CD3 (UCHT1) Mouse IgG2a Recombinant Antibody (CPY5-65570, Clone:UCHT1)(red), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

CD3 Recombinant Antibody for FC

Cat No. CPY5-65570

Clone No.UCHT1

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

CD3E, CD3 epsilon, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, T3E

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CPY5-65570 targets CD3 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor

• Full Name: CD3e molecule, epsilon (CD3-TCR complex)
• Calculated Molecular Weight: 207 aa, 23 kDa
• GenBank Accession Number: BC049847
• Gene Symbol: CD3
• Gene ID (NCBI): 916
• ENSEMBL Gene ID: ENSG00000198851
• RRID: AB_3673826
• Conjugate: PerCP-Cyanine5.5 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 482 nm / 690nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P07766
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).