CoraLite® Plus 647 Anti-Mouse CXCL2 Rabbit Recombinant Antibody

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  FC experiment of RAW 264.7 using CL647-98259

  1x10^6 untreated or LPS and Brefeldin A treated RAW 264.7 cells were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CXCL2 Rabbit RecAb (CL647-98259, Clone: 242007G5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of RAW 264.7 using CL647-98259

  1x10^6 LPS and Brefeldin A treated RAW 264.7 cells were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CXCL2 Rabbit RecAb (CL647-98259, Clone: 242007G5) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). 1x10^6 untreated RAW 264.7 cells were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CXCL2 Rabbit RecAb (CL647-98259, Clone: 242007G5) (black, dashed). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CXCL2 Recombinant Antibody for FC (Intra)

Cat No. CL647-98259

Clone No.242007G5

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

242007G5, C-X-C motif chemokine 2, Macrophage inflammatory protein 2, MIP2, Mip-2

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Tested Applications

• Positive FC (Intra) detected in: LPS and Brefeldin A treated RAW 264.7 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98259 targets CXCL2 in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CXCL2 fusion protein Eg2239
• Full Name: chemokine (C-X-C motif) ligand 2
• Calculated Molecular Weight: 11 kDa
• GenBank Accession Number: NM_009140
• Gene Symbol: Cxcl2
• Gene ID (NCBI): 20310
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P10889
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CXCL2 is a member of the CXC subfamily, which encodes secreted proteins involved in immunoregulatory and inflammatory processes. CXCL2 is expressed at sites of inflammation and may suppress hematopoietic progenitor cell proliferation. CXCL2 plays an critical role in maintaining cancer-induced macrophage infiltration and the resulting mechanical hypersensitivity and persistent spontaneous nociception (PMID: 36754246).