Mouse-IgG Polyclonal antibody

Mouse-IgG Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IP, CoIP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Publications

31

Cat no : 10283-1-AP

Synonyms

Mouse IgG

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• Validation Data Gallery
• Proteintech vs. Other Vendors

• 

  WB analysis of mouse serum using 10283-1-AP

  mouse serum were subjected to SDS PAGE followed by western blot with 10283-1-AP (Mouse-IgG antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse serum tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below
• ChIP: See 1 publications below
• RIP: See 4 publications below

Product Information

10283-1-AP targets Mouse-IgG in WB, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: Mouse-IgG
• Calculated Molecular Weight: 155 kDa
• Observed Molecular Weight: 25 kDa, 50-55 kDa
• RRID: AB_2877728
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody detectes all the heavy and light chains total mouse IgG.

Publications

Species	Application	Title
		Nucleic Acids Res; An H3K4me3 reader, BAP18 as an adaptor of COMPASS-like core subunits co-activates ERα action and associates with the sensitivity of antiestrogen in breast cancer.; Authors - Ge Sun
	WB	Comput Struct Biotechnol J; Impact of Cuproptosis-related markers on clinical status, tumor immune microenvironment and immunotherapy in colorectal cancer: A multi-omic analysis; Authors - Yanfei Shao
		Cell Physiol Biochem; Long-Noncoding RNA Colorectal Neoplasia Differentially Expressed Gene as a Potential Target to Upregulate the Expression of IRX5 by miR-136-5P to Promote Oncogenic Properties in Hepatocellular Carcinoma.; Authors - Liying Zhu
	IP	J Clin Endocrinol Metab; TRIM65 Promotes Invasion of Endometrial Stromal Cells by Activating ERK1/2/C-myc Signaling via Ubiquitination of DUSP6.; Authors - Ying-Ting Wu
		Int J Mol Sci; Social Learning of Acquiring Novel Feeding Habit in Mandarin Fish (Siniperca chuatsi).; Authors - Jian Peng
		Sci Rep; Premature termination codon readthrough in Drosophila varies in a developmental and tissue-specific manner.; Authors - Yanan Chen