Rabbit anti-Mouse IgG Polyclonal antibody

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• Rabbit anti-Mouse IgG Polyclonal antibody (10283-1-AP)

Rabbit anti-Mouse IgG Polyclonal Antibody for WB, IP, ELISA

Cat No. 10283-1-AP

Publications(43)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IP, CoIP, ChIP, RIP, ELISA

Synonyms

Mouse-IgG, anti mouse igg, anti-mouse igg, Mouse IgG

• Validation Data Gallery
• Proteintech vs. Other Vendors

• 

  WB analysis of mouse serum using 10283-1-AP

  mouse serum were subjected to SDS PAGE followed by western blot with 10283-1-AP (Rabbit anti-Mouse IgG antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse serum using 10283-1-AP

  IP result of Rabbit anti-Mouse IgG (IP:10283-1-AP, 4ug; Detection:10283-1-AP 1:10000) with mouse serum lysate 2200 ug.

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Tested Applications

• Positive WB detected in: mouse serum tissue
• Positive IP detected in: mouse serum

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below
• IP: See 4 publications below
• CoIP: See 2 publications below
• ChIP: See 2 publications below
• RIP: See 6 publications below

Product Information

10283-1-AP targets Rabbit anti-Mouse IgG in WB, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: Rabbit anti-Mouse IgG
• Calculated Molecular Weight: 155 kDa
• Observed Molecular Weight: 25 kDa, 50-55 kDa
• RRID: AB_2877728
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody detectes all the heavy and light chains total mouse IgG.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Nucleic Acids Res; An H3K4me3 reader, BAP18 as an adaptor of COMPASS-like core subunits co-activates ERα action and associates with the sensitivity of antiestrogen in breast cancer.; Authors - Ge Sun
	WB	Comput Struct Biotechnol J; Impact of Cuproptosis-related markers on clinical status, tumor immune microenvironment and immunotherapy in colorectal cancer: A multi-omic analysis; Authors - Yanfei Shao
	RIP	Cancer Sci; LncRNA-DANCR Promotes ESCC Progression and Function as ceRNA to Regulate DDIT3 Expression by Sponging microRNA-3193; Authors - Heng Xiao
		Cell Physiol Biochem; Long-Noncoding RNA Colorectal Neoplasia Differentially Expressed Gene as a Potential Target to Upregulate the Expression of IRX5 by miR-136-5P to Promote Oncogenic Properties in Hepatocellular Carcinoma.; Authors - Liying Zhu
	IP	J Clin Endocrinol Metab; TRIM65 Promotes Invasion of Endometrial Stromal Cells by Activating ERK1/2/C-myc Signaling via Ubiquitination of DUSP6.; Authors - Ying-Ting Wu
		Int J Mol Sci; Social Learning of Acquiring Novel Feeding Habit in Mandarin Fish (Siniperca chuatsi).; Authors - Jian Peng