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Rabbit anti-Mouse IgG Polyclonal antibody

Rabbit anti-Mouse IgG Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IP, CoIP, ChIP, RIP, ELISA
For IF, select conjugated version.

Mouse-IgG, anti mouse igg, anti-mouse igg, Mouse IgG

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Size/Concentration: 
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  • Western Blot (WB) analysis of mouse serum using Rabbit anti-Mouse IgG Polyclonal antibody (10283-1-AP)

    WB analysis of mouse serum using 10283-1-AP

    mouse serum were subjected to SDS PAGE followed by western blot with 10283-1-AP (Rabbit anti-Mouse IgG antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunoprecipitation (IP) experiment of mouse serum using Rabbit anti-Mouse IgG Polyclonal antibody (10283-1-AP)

    IP experiment of mouse serum using 10283-1-AP

    IP result of Rabbit anti-Mouse IgG (IP:10283-1-AP, 4ug; Detection:10283-1-AP 1:10000) with mouse serum lysate 2200 ug.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

"Rabbit anti-Mouse IgG Antibodies" Comparison

View side-by-side comparison of Rabbit anti-Mouse IgG antibodies from other vendors to find the one that best suits your research needs.
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What's the best dilution for Rabbit anti-Mouse IgG in ?

Tested Applications

Positive WB detected inmouse serum tissue
Positive IP detected inmouse serum

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10283-1-AP targets Rabbit anti-Mouse IgG in WB, IHC, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

Predict reactive species
Full Name Rabbit anti-Mouse IgG
Calculated Molecular Weight 155 kDa
Observed Molecular Weight 25 kDa, 50-55 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_2877728
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody detectes all the heavy and light chains total mouse IgG.

Protocols

Product Specific Protocols
WB protocol for Rabbit anti-Mouse IgG antibody 10283-1-APDownload protocol
IP protocol for Rabbit anti-Mouse IgG antibody 10283-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The Rabbit anti-Mouse IgG Polyclonal antibody (Cat# 10283-1-AP) has 44 citations published in journals including Nucleic Acids Research, Journal of Clinical Investigation, Cell Death & Differentiation, Cell Reports, Advanced Science, Journal of Biological Chemistry, and Cancer Science. Research fields span cancer biology (lung, breast, ovarian, colorectal, prostate), epigenetics and chromatin biology, non-coding RNA regulation, fibrosis, circadian biology, immunology, neurology, toxicology, and virology.

SpeciesApplicationTitle

Nucleic Acids Res

SCRE serves as a unique synaptonemal complex fastener and is essential for progression of meiosis prophase I in mice.

Authors - Hongbin Liu

Nucleic Acids Res

An H3K4me3 reader, BAP18 as an adaptor of COMPASS-like core subunits co-activates ERα action and associates with the sensitivity of antiestrogen in breast cancer.

Authors - Ge Sun
mouseChIP

J Clin Invest

Excessive collagen type VII mediates pleural fibrosis via increasing extracellular matrix stiffness.

Authors - Qian Li
RIP

Adv Sci (Weinh)

The Circadian Transcription Factor CLOCK Modulates Oxidative Stress Resistance via the ACHL-Relish Axis in Drosophila.

Authors - Xu Liu

Cell Death Differ

KMT2D loss drives adeno-to-squamous transition and sensitizes TKI-resistant lung cancer to AURKA inhibition

Authors - Nana Chen
WB

Cell Death Dis

CDCA8 promotes bladder cancer survival by stabilizing HIF1α expression under hypoxia

Authors - Qiang Zhou
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