CoraLite®594-conjugated Rabbit anti-Mouse IgG Polyclonal antibody

• 

  IF Staining of HeLa using CL594-10283

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66031-1-Ig (alpha tubulin antibody) and CL594-10283 (CL594-Rabbit anti-Mouse IgG) as secondary antibody at dilution of 1:200.

Rabbit anti-Mouse IgG Polyclonal Antibody for IF/ICC

Cat No. CL594-10283

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF/ICC

Synonyms

Mouse-IgG, anti mouse igg, anti-mouse igg, Mouse IgG

---

Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL594-10283 targets Rabbit anti-Mouse IgG in IF/ICC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: Rabbit anti-Mouse IgG
• Calculated Molecular Weight: 155 kDa
• Observed Molecular Weight: 25 kDa, 50-55 kDa
• RRID: AB_2919726
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Excitation Laser: Yellow-Green Laser (561 nm)
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

This antibody detectes all the heavy and light chains of total mouse IgG.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Adv Sci (Weinh); Understanding Glioblastoma Dynamics Using 3D Organoids and Engineered Extracellular Matrix.; Authors - Autumn Mcmanis
	IF	bioRxiv; Understanding proneural-mesenchymal transition using patient-derived glioma stem-like cell (GSC) organoids and engineered extracellular matrix.; Authors - Autumn Mcmanis