mouse serum were subjected to SDS PAGE followed by western blot with HRP-10283 (Mouse-IgG antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
mouse serum were subjected to SDS PAGE followed by western blot with HRP-10283 (Mouse-IgG antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Tested Applications
Positive WB detected in
mouse serum
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
HRP-10283 targets Mouse-IgG in WB applications and shows reactivity with mouse samples.
Tested Reactivity
mouse
Host / Isotype
Rabbit / IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Protein
Full Name
Mouse-IgG
Calculated Molecular Weight
155 kDa
Observed Molecular Weight
25 kDa, 50-55 kDa
Gene Symbol
Gene ID (NCBI)
Conjugate
HRP
Form
Liquid
Purification Method
Antigen affinity purification
Storage Buffer
PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage Conditions
Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
This antibody detectes all the heavy and light chains total mouse IgG.
mouse serum were subjected to SDS PAGE followed by western blot with HRP-10283 (Mouse-IgG antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Mouse-IgG Polyclonal antibody
Catalog Number
HRP-10283
Citations
-
Dilutions
WB : 1:2000-1:10000
Applications
WB
Reactivity
mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet