Biotin-conjugated Rabbit anti-Mouse IgG Polyclonal antibody

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  IHC staining of human colon cancer using Biotin-10283

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66031-1-Ig (alpha tubulin antibody) and Biotin-10283 (Biotin-Rabbit anti-Mouse IgG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Rabbit anti-Mouse IgG Polyclonal Antibody for IHC, ELISA

Cat No. Biotin-10283

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IHC, ELISA

Synonyms

Mouse-IgG, anti mouse igg, anti-mouse igg, Mouse IgG

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Tested Applications

• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-10283 targets Rabbit anti-Mouse IgG in IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: Rabbit anti-Mouse IgG
• Calculated Molecular Weight: 155 kDa
• Observed Molecular Weight: 25 kDa, 50-55 kDa
• RRID: AB_2918932
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

This antibody detects all the heavy and light chains of total mouse IgG.