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What's the best dilution for Rabbit anti-Mouse IgG in ?
Tested Applications
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-10283 targets Rabbit anti-Mouse IgG in IF/ICC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | Rabbit anti-Mouse IgG |
| Calculated Molecular Weight | 155 kDa |
| Observed Molecular Weight | 25 kDa, 50-55 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2918969 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
This antibody detectes all the heavy and light chains total mouse IgG.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Rabbit anti-Mouse IgG antibody CL488-10283 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CoraLite® Plus 488-conjugated Rabbit anti-Mouse IgG Polyclonal antibody (Cat# CL488-10283) has a total of 53 citations. One indexed citation appears in Biochemical and Biophysical Research Communications (2025), studying alkylglycerol-enhanced myogenesis and ether-phospholipid metabolism in C2C12 myoblasts using immunofluorescence. The research fields encompass muscle biology, lipid metabolism, and cell differentiation.
| Species | Application | Title |
|---|---|---|
Biochem Biophys Res Commun Alkylglycerol enhances myogenesis and regulates ether-phospholipid metabolism in C2C12 myoblasts |
