CoraLite® Plus 647-conjugated Mouse-IgG Polyclonal antibody

Mouse-IgG Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-10283

Synonyms

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Validation Data Gallery

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  IF Staining of HeLa using CL647-10283

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66031-1-Ig (alpha tubulin antibody) and CL647-10283 (CL647-Rabbit anti-Mouse IgG as secondary antibody at dilution of 1:200.

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Tested Applications

• Positive IF detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-10283 targets Mouse-IgG in IF applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: Mouse-IgG
• Calculated Molecular Weight: 155 kDa
• Observed Molecular Weight: 25 kDa, 50-55 kDa
• RRID: AB_2920210
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody detectes all the heavy and light chains of total mouse IgG.