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CoraLite® Plus 488-conjugated PEBP1 Monoclonal antibody

PEBP1 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D4D11

Cat no : CL488-66438

Synonyms

HCNP, HCNPpp, Neuropolypeptide h3, PBP, PEBP, PEBP 1, PEBP1, Prostatic binding protein, Raf kinase inhibitor protein, RKIP

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite® Plus 488-conjugated PEBP1 Monoclonal ant (CL488-66438)

    IF Staining of A549 using CL488-66438

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using CoraLite® Plus 488 PEBP1 antibody (CL488-66438, Clone: 2D4D11 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of PC-12 cells using CoraLite® Plus 488-conjugated PEBP1 Monoclonal ant (CL488-66438)

    FC experiment of PC-12 using CL488-66438

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PEBP1 (CL488-66438, Clone:2D4D11) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inA549 cells
Positive FC detected inPC-12 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66438 targets PEBP1 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PEBP1 fusion protein Ag0864
Full Name phosphatidylethanolamine binding protein 1
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 23-27 kDa
GenBank Accession NumberBC008714
Gene Symbol PEBP1
Gene ID (NCBI) 5037
RRIDAB_2919325
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Raf kinase inhibitor protein (RKIP), also termed phosphatidylethanolamine binding protein PEBP1, was initially identified as a potent inhibitor of Raf-1/MEK/ERK, NF-kB, and G-protein-coupled receptor signaling pathways. Later RKIP has been further identified as a metastasis suppressor and its loss of expression has been reported in various cancers. Its expression has been proposed as a prognostic marker for patients diagnosed with the above cancers.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PEBP1 antibody CL488-66438Download protocol
FC protocol for CL Plus 488 PEBP1 antibody CL488-66438Download protocol
Standard Protocols
Click here to view our Standard Protocols