CoraLite®594-conjugated PGRMC2 Monoclonal antibody

• Featured Product
• KD/KO Validated

PGRMC2 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5F10D7

Cat no : CL594-60249

Synonyms

DG6, PGRMC2, PMBP

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Validation Data Gallery

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  IF Staining of human cervical cancer using CL594-60249

  Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using CoraLite®594 PGRMC2 antibody (CL594-60249, Clone: 5F10D7 ) at dilution of 1:200.

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  IF Staining of human cervical cancer using CL594-60249

  Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using CoraLite®594 PGRMC2 antibody (CL594-60249, Clone: 5F10D7 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: human cervical cancer tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL594-60249 is PGRMC2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: PGRMC2 fusion protein Ag20077
• Full Name: progesterone receptor membrane component 2
• Calculated Molecular Weight: 223 aa, 24 kDa
• Observed Molecular Weight: 24+28 kDa
• GenBank Accession Number: BC016692
• Gene Symbol: PGRMC2
• Gene ID (NCBI): 10424
• RRID: AB_2919905
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Membrane-associated progesterone receptor component 2 (PGRMC2), also known as DG6 or PMBP, belongs to the cytochrome b5 family. This protein might play a role in cancer. The gene of PGRMC2 maps to chromosome 4q26, and encodes a 223-amino acid single-pass membrane protein with a cytochrome b5 heme-binding domain in its cytoplasmic domain. PGRMC2 has a calculated molecular weight of 24 kDa. The band of about 28 kDa detected by this monoclonal antibody is unknown but may represent phosphorylated form of PGRMC2 (PMID: 28104494).