CoraLite® Plus 594-conjugated ki67 Polyclonal antibody

ki67 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF

Conjugate

CoraLite® Plus 594 Fluorescent Dye

Cat no : CL594-28074

Synonyms

D630048A14Rik, Ki 67, Ki67, Mki67

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Validation Data Gallery

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  IF Staining of mouse colon using CL594-28074

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CoraLite® Plus 594 ki67 antibody (CL594-28074) at dilution of 1:200.

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  IF Staining of mouse colon using CL594-28074

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CoraLite® Plus 594 ki67 antibody (CL594-28074) at dilution of 1:200.

• 

  IF Staining of mouse spleen using CL594-28074

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite® Plus 594 ki67 antibody (CL594-28074) at dilution of 1:200.

• 

  IF Staining of mouse spleen using CL594-28074

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite® Plus 594 ki67 antibody (CL594-28074) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: mouse colon tissue, mouse spleen tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-28074 targets ki67 in IF applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ki67 fusion protein Ag27894
• Full Name: antigen identified by monoclonal antibody Ki 67
• Calculated Molecular Weight: 351 kDa
• GenBank Accession Number: NM_001081117
• Gene Symbol: Mki67
• Gene ID (NCBI): 17345
• Conjugate: CoraLite® Plus 594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 594 nm / 615 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry.