ChromoTek GFP-Trap® Dynabeads

GFP-Trap® Dynabeads for immunoprecipitation (IP) of GFP-tagged proteins. GFP-Trap® Dynabeads is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a GFP VHH/ Nanobody coupled to Dynabeads™.

Ready to use reagent

Specificity

GFP, eGFP, CFP, YFP, BFP

Applications

IP, CoIP, ChIP, RIP

Conjugate

Dynabeads

Publications

5

Cat no : gtd

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Trap, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet

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Validation Data Gallery

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  Immunoprecipitation (IP) of GFP with GFP-Trap Dynabeads. I: Input, FT: Flow-Through, B: Bound.

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  Immunoprecipitation (IP) of GFP with GFP-Trap Dynabeads: Coomassie and Western blot (I: Input, FT: Flow-Through, B: Bound).

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  Cartoon to visualize the binding of large GFP-fusion proteins (GFP (green) + protein of interest (POI, blue) with interacting partner X (Prot X, orange) or multimeric proteins (GFP (green) + protein of interest (POI, blue) to the GFP VHH (dark green) of GFP-Trap Magnetic Agarose or GFP-Trap Dynabeads.

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Product Information

GFP-Trap® Dynabeads for immunoprecipitation (IP) of GFP-tagged proteins. GFP-Trap® Dynabeads is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a GFP VHH/ Nanobody coupled to Dynabeads™.

• Applications: IP, CoIP, ChIP, RIP
• Clonality: Monoclonal
• Host: Alpaca
• Affinity: Dissociation constant KD of 1 pM
• Specificity/Target: AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP
• Coupled VHH: Monoclonal anti-Green Fluorescent Protein (GFP) single domain antibody (sdAb) fragment, recombinantly expressed
• Conjugate: Dynabeads
• Beads: ~ 2.8 µm (Magnetic, high throughput-compatible)
• Binding capacity: 10 µL slurry bind about 1 µg of recombinant GFP
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Storage Buffer: 1x PBS, Preservative: 0.09 % sodium azide
• Storage Condition: Upon receipt store at +4°C. Do not freeze!

Publications

Application	Title
IP	Nat Commun; SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.; Authors - Seiichi Koike
IP	Cell Rep; WDR82/PNUTS-PP1 Prevents Transcription-Replication Conflicts by Promoting RNA Polymerase II Degradation on Chromatin.; Authors - Helga B Landsverk
IP	J Biol Chem; Leptin modulates pancreatic β-cell membrane potential through Src kinase-mediated phosphorylation of NMDA receptors.; Authors - Veronica A Cochrane
IP	Open Biol; Coordinated regulation of the ESCRT-III component CHMP4C by the chromosomal passenger complex and centralspindlin during cytokinesis.; Authors - Luisa Capalbo
IP	Genes Dev; Argonaute NRDE-3 and MBT domain protein LIN-61 redundantly recruit an H3K9me3 HMT to prevent embryonic lethality and transposon expression.; Authors - Jan Padeken