ChromoTek GFP-Trap® Dynabeads
GFP-Trap® Dynabeads for immunoprecipitation (IP) of GFP-tagged proteins. GFP-Trap® Dynabeads is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a GFP VHH/ Nanobody coupled to Dynabeads™.
Ready to use reagent
Specificity
GFP, eGFP, CFP, YFP, BFP
Applications
IP, CoIP, ChIP, RIP
Conjugate
Dynabeads
Cat no : gtd
Synonyms
Validation Data Gallery
Product Information
GFP-Trap® Dynabeads for immunoprecipitation (IP) of GFP-tagged proteins. GFP-Trap® Dynabeads is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a GFP VHH/ Nanobody coupled to Dynabeads™.
Applications | IP, CoIP, ChIP, RIP |
Clonality | Monoclonal |
Host | Alpaca |
Affinity | Dissociation constant KD of 1 pM |
Specificity/Target | AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP |
Coupled VHH | Monoclonal anti-Green Fluorescent Protein (GFP) single domain antibody (sdAb) fragment, recombinantly expressed |
Conjugate | Dynabeads |
Beads | ~ 2.8 µm (Magnetic, high throughput-compatible) |
Binding capacity | 10 µL slurry bind about 1 µg of recombinant GFP |
Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
Storage Buffer | 1x PBS, Preservative: 0.09 % sodium azide |
Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Documentation
SDS |
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SDS GFP-Trap Dynabeads |
Protocol |
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Protocol GFP-Trap Dynabeads?(PDF) |
Brochure |
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GFP-Trap product brochure |
GFP-Trap Specificity |
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Fluorescent protein specificity table |
Trouble shooting |
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Troubleshooting guide immunoprecipitation (IP) |
White papers |
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How to plan an Immunoprecipitation using the GFP-Trap |
GFP-Trap? as affinity tag |
Unique Stability of the ChromoTek GFP-Trap:GFP Complex |
Split Fluorescent Protein Technology (PDF) |
Publications
Application | Title |
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Nat Commun SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors. | |
Cell Rep WDR82/PNUTS-PP1 Prevents Transcription-Replication Conflicts by Promoting RNA Polymerase II Degradation on Chromatin. | |
J Biol Chem Leptin modulates pancreatic β-cell membrane potential through Src kinase-mediated phosphorylation of NMDA receptors. | |
Open Biol Coordinated regulation of the ESCRT-III component CHMP4C by the chromosomal passenger complex and centralspindlin during cytokinesis. | |
Genes Dev Argonaute NRDE-3 and MBT domain protein LIN-61 redundantly recruit an H3K9me3 HMT to prevent embryonic lethality and transposon expression. |