ChromoTek GFP-Trap® Dynabeads

GFP-Trap® Dynabeads for immunoprecipitation (IP) of GFP-tagged proteins. GFP-Trap® Dynabeads is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a GFP VHH/ Nanobody coupled to Dynabeads™.

Ready to use reagent

Specificity

GFP, eGFP, CFP, YFP, BFP

Applications

IP, CoIP, ChIP, RIP

Conjugate

Dynabeads

Cat no : gtd

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Trap, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet



Product Information

GFP-Trap® Dynabeads for immunoprecipitation (IP) of GFP-tagged proteins. GFP-Trap® Dynabeads is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a GFP VHH/ Nanobody coupled to Dynabeads™.

ApplicationsIP, CoIP, ChIP, RIP
Clonality Monoclonal
Host Alpaca
AffinityDissociation constant KD of 1 pM
Specificity/TargetAcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP
Coupled VHHMonoclonal anti-Green Fluorescent Protein (GFP) single domain antibody (sdAb) fragment, recombinantly expressed
ConjugateDynabeads
Beads~ 2.8 µm (Magnetic, high throughput-compatible)
Binding capacity10 µL slurry bind about 1 µg of recombinant GFP
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Storage Buffer1x PBS, Preservative: 0.09 % sodium azide
Storage Condition Upon receipt store at +4°C. Do not freeze!

Publications

ApplicationTitle
IP

Nat Commun

SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.

Authors - Seiichi Koike
IP

Cell Rep

WDR82/PNUTS-PP1 Prevents Transcription-Replication Conflicts by Promoting RNA Polymerase II Degradation on Chromatin.

Authors - Helga B Landsverk
IP

J Biol Chem

Leptin modulates pancreatic β-cell membrane potential through Src kinase-mediated phosphorylation of NMDA receptors.

Authors - Veronica A Cochrane
IP

Open Biol

Coordinated regulation of the ESCRT-III component CHMP4C by the chromosomal passenger complex and centralspindlin during cytokinesis.

Authors - Luisa Capalbo
IP

Genes Dev

Argonaute NRDE-3 and MBT domain protein LIN-61 redundantly recruit an H3K9me3 HMT to prevent embryonic lethality and transposon expression.

Authors - Jan Padeken