ChromoTek GFP-Trap® Magnetic Agarose
The ChromoTek GFP-Trap® Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. It comprises a GFP Nanobody/ VHH coupled to magnetic agarose beads.
Specificity
GFP, eGFP, CFP, YFP, BFP
Applications
IP, CoIP, ChIP, RIP
Conjugate
Magnetic Agarose
900
Cat no : gtma
Synonyms
Validation Data Gallery
Product Information
The ChromoTek GFP-Trap® Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. It comprises a GFP Nanobody/ VHH coupled to magnetic agarose beads.
Applications | IP, CoIP, ChIP, RIP |
Clonality | Monoclonal |
Host | Alpaca |
Affinity | Dissociation constant KD of 1 pM |
Specificity/Target | AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP |
Coupled VHH | Recombinant, monoclonal anti-Green Fluorescent Protein (GFP) single domain antibody (sdAb) fragment |
Conjugate | Magnetic Agarose |
Beads | ~ 40 µm |
Binding capacity | 10 µL slurry bind more than 8 µg of recombinant GFP |
Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
Storage Buffer | 20% EtOH |
Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Documentation
SDS |
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SDS GFP-Trap_Magnetic Agarose |
Protocol |
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GFP-Trap 磁性琼脂糖珠操作说明(中文) |
Protocol GFP-Trap_Magnetic Agarose (PDF) |
White papers |
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How to plan an Immunoprecipitation using the GFP-Trap |
Split Fluorescent Protein Technology (PDF) |
GFP-Trap? as affinity tool |
Unique Stability of the ChromoTek GFP-Trap:GFP Complex |
GFP-Trap Specificity |
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Fluorescent protein specificity table |
Trouble shooting |
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Troubleshooting guide immunoprecipitation (IP) |
Brochure |
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GFP-Trap product brochure |
Publications
Application | Title |
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Mol Cell Spatiotemporal Control of ULK1 Activation by NDP52 and TBK1 during Selective Autophagy. | |
Mol Cell Genome-wide CRISPR Analysis Identifies Substrate-Specific Conjugation Modules in ER-Associated Degradation. | |
Mol Cell Reciprocal Roles of Tom7 and OMA1 during Mitochondrial Import and Activation of PINK1. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH James (Verified Customer) (06-16-2022) | Overexpressed GFP in HEK293T cells - efficient pulldown with GFP beads.
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