ChromoTek GFP-Trap® Magnetic Agarose

The ChromoTek GFP-Trap® Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. It comprises a GFP Nanobody/ VHH coupled to magnetic agarose beads.

Specificity

GFP, eGFP, CFP, YFP, BFP

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic Agarose

Publications

900

Reviews (1)

Cat no : gtma

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Trap, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet

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Validation Data Gallery

• 

  GFP-Trap Magnetic Agarose for immunoprecipitation of GFP-fusion proteins. Note single-band in bound fraction, which indicates a very effective pull-down. I: Input, FT: Flow-Through, B: Bound.

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Product Information

The ChromoTek GFP-Trap® Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. It comprises a GFP Nanobody/ VHH coupled to magnetic agarose beads.

• Applications: IP, CoIP, ChIP, RIP
• Clonality: Monoclonal
• Host: Alpaca
• Affinity: Dissociation constant KD of 1 pM
• Specificity/Target: AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP
• Coupled VHH: Recombinant, monoclonal anti-Green Fluorescent Protein (GFP) single domain antibody (sdAb) fragment
• Conjugate: Magnetic Agarose
• Beads: ~ 40 µm
• Binding capacity: 10 µL slurry bind more than 8 µg of recombinant GFP
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Storage Buffer: 20% EtOH
• Storage Condition: Upon receipt store at +4°C. Do not freeze!

Publications

Application	Title
IP	Dev Cell; Phosphorylation of Syntaxin 17 by TBK1 Controls Autophagy Initiation.; Authors - Suresh Kumar
IP	Mol Cell; Spatiotemporal Control of ULK1 Activation by NDP52 and TBK1 during Selective Autophagy.; Authors - Jose Norberto S Vargas
IP	Dev Cell; FAT4 Fine-Tunes Kidney Development by Regulating RET Signaling.; Authors - Hongtao Zhang
CoIP	Mol Cell; Genome-wide CRISPR Analysis Identifies Substrate-Specific Conjugation Modules in ER-Associated Degradation.; Authors - Dara E Leto
IP	Sci Adv; RAB7A phosphorylation by TBK1 promotes mitophagy via the PINK-PARKIN pathway.; Authors - J-M Heo
IP	Mol Cell; Reciprocal Roles of Tom7 and OMA1 during Mitochondrial Import and Activation of PINK1.; Authors - Shiori Sekine

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

James (Verified Customer) (06-16-2022)

Overexpressed GFP in HEK293T cells - efficient pulldown with GFP beads.

• Applications: Immunoprecipitation
• Cell Tissue Type: HEK293T