ChromoTek GFP-Trap® Magnetic Agarose

Validation Data Gallery

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  GFP-Trap Magnetic Agarose for immunoprecipitation of GFP-fusion proteins. Note single-band in bound fraction, which indicates a very effective pull-down. I: Input, FT: Flow-Through, B: Bound.

The ChromoTek GFP-Trap® Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. It comprises a GFP Nanobody/ VHH coupled to magnetic agarose beads.

Cat No. gtma

Publications(1459)

Reviews (13)

Specificity

GFP and GFP derivates

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Conjugate

Magnetic agarose

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Trap, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet

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Product Information

The ChromoTek GFP-Trap® Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. It comprises a GFP Nanobody/ VHH coupled to magnetic agarose beads.

• Description: Immunoprecipitation of GFP-fusion proteins and their interacting factors with anti-GFP Nanobody conjugated to magnetic agarose beads.

  • Fast, reliable & efficient one-step immunoprecipitation

  • Ready-to-use

  • No heavy & light antibody chains

  • Stable under harsh washing conditions

  • Suitable for downstream mass spec analysis

  • Works in samples from: mammals, plants, bacteria, yeast, insects etc.

• Applications: IP, CoIP, ChIP, RIP
• Specificity/Target: AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP ; For the complete list, please click here: Fluorescent protein specificity table
• Binding capacity: 15-20 μg of recombinant GFP per 25 μL bead slurry
• Conjugate: Magnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads)
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Wash buffer compatibility: 1 mM DTT, 3 M Guanidinium•HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 1 pM
• Compatibility with mass spectrometry: The GFP-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
• RRID: AB_2631358
• Storage Buffer: 20% ethanol
• Storage Condition: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!
• Size: 25ul/reactions (eg：20rxns=500ul slurry)

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Publications

Application	Title
IP	Nature; Polθ is phosphorylated by PLK1 to repair double-strand breaks in mitosis; Authors - Camille Gelot
CoIP	Nature; Reducing brassinosteroid signalling enhances grain yield in semi-dwarf wheat; Authors - Long Song
IP	Cell; STING orchestrates the neuronal inflammatory stress response in multiple sclerosis; Authors - Marcel S Woo
ChIP	Cell; Chromatin remodeling of histone H3 variants by DDM1 underlies epigenetic inheritance of DNA methylation; Authors - Seung Cho Lee
IP	Cell; Pathogen protein modularity enables elaborate mimicry of a host phosphatase; Authors - Hui Li
IP	Nature; A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol.; Authors - Evelyn Fessler

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Maurizio (Verified Customer) (04-30-2025)

Excellent reagent. The pull-down efficiency of the GFP-Trap system is way higher than the one obtained with standard anti-GFP antibodies.

• Applications: Immunoprecipitation
• Cell Tissue Type: HeLa cells

FH

Timo (Verified Customer) (01-30-2025)

CoIP resulted in a very clean result and the technical support was excellent. Will 100% recommend!

• Applications: Immunoprecipitation
• Cell Tissue Type: Yeast

FH

Karel (Verified Customer) (11-20-2024)

We achieved very nice recovery of protein of interest.

• Applications: ChIP
• Cell Tissue Type: yeast

FH

Haifei (Verified Customer) (09-13-2024)

Easily followed protocol. Clean results.

• Applications: Immunoprecipitation
• Cell Tissue Type: 293T

FH

Alen (Verified Customer) (08-20-2024)

Great and convenient product

• Applications: Immunoprecipitation
• Cell Tissue Type: Protein

FH

Margaux (Verified Customer) (06-11-2024)

I used GFP-TRAP to isolate GFP-tagged proteins. It was very efficient and it's a real advantage not to have to deal with heavy and light antibody chains.

• Applications: Immunoprecipitation
• Cell Tissue Type: HeLa cells

FH

Titouan (Verified Customer) (06-11-2024)

Very efficient pull-down of GFP-tagged protein from cell lysates (293T, Hela...) and very easy to use

• Applications: Immunoprecipitation
• Cell Tissue Type: HEK293T

FH

Mona (Verified Customer) (08-03-2023)

I purified GF tagged protein and efficiency was almost 100%

• Applications: Protein purification
• Cell Tissue Type: HeLa cell line

FH

Rhys (Verified Customer) (11-08-2022)

Used for IP of a eGFP tagged construct, easy to use and excellent efficiency.

• Applications: Immunoprecipitation

FH

Peter (Verified Customer) (10-24-2022)

Best system I have used for GFP IPs

• Applications: Immunoprecipitation
• Cell Tissue Type: U2OS

FH

Amy (Verified Customer) (09-02-2022)

Efficient pull down of GFP-tagged protein from HEK293T cell lysates.

• Applications: Immunoprecipitation

FH

Rich (Verified Customer) (07-26-2022)

• Applications: Western Blot,Immunoprecipitation
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: liver

FH

James (Verified Customer) (06-16-2022)

Overexpressed GFP in HEK293T cells - efficient pulldown with GFP beads.

• Applications: Immunoprecipitation
• Cell Tissue Type: HEK293T