Tested Applications
| Positive IF-P detected in | mouse heart tissue |
| Positive IF-Fro detected in | mouse small intestine tissue |
| Positive IF/ICC detected in | H9C2 cells |
| Positive FC (Intra) detected in | C2C12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-80008 targets Alpha smooth muscle actin specific in IF/ICC, IF-P, IF-Fro, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, chicken samples.
| Tested Reactivity | human, mouse, rat, pig, chicken |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | actin, alpha 2, smooth muscle, aorta |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | NM_001613 |
| Gene Symbol | Alpha smooth muscle actin |
| Gene ID (NCBI) | 59 |
| RRID | AB_2923883 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P62736 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 Alpha smooth muscle actin specific antibody CL488-80008 | Download protocol |
| IF protocol for CL Plus 488 Alpha smooth muscle actin specific antibody CL488-80008 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The α-smooth muscle actin specific recombinant monoclonal antibody (5H7), catalog number CL488-80008, has 2 citations. These appear in Cell and J Orthop Translat. The research fields include breast cancer tumor microenvironment and immune exclusion, as well as spinal cord injury and fibrotic pericyte-to-myofibroblast transition.
| Species | Application | Title |
|---|---|---|
Cell Sensory neurons drive immune exclusion by stimulating a dense extracellular matrix in the breast cancer tumor microenvironment. | ||
J Orthop Translat Profibrotic macrophage-derived CXCL4 promotes pericyte-to-myofibroblast transition after spinal cord injury. |
