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IFT88 Antibody

KD/KO Validated

KD/KO Validated

Antibody Catalog number Type Applications  
IFT88 13967-1-AP Rabbit Poly ELISA, IF, IHC, IP, WB 194 Publications

Among its many functions, IFT88 helps mediate intraflagellar transport, which is essential for the assembly and maintenance of cilia and flagella in many organisms. The Proteintech polyclonal IFT88 antibody has appeared in over 190 publications to date.

One of these is related to Kif3 protein, and its role in mammalian Hedgehog (Hh) signaling, where researchers used anti‑IFT88 antibody as a marker for cilium retraction along with acetylated tubulin in IF staining.¹

IFT88 antibody validated in Immunofluorescent IFT88 antibody validated in western blot

Immunofluorescent images of MDCK cells stained with IFT88 rabbit pAb (13967-1-AP) and acetylated tubulin mouse mAb (66200-1-Ig) at a dilution of 1:50, further stained with Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) for 13967-1-AP, and Alexa Fluor 488-conjugated AffiniPure Goat anti-Mouse IgG (H+L) for 66200-1-Ig

HEK-293 cells were subjected to SDS PAGE followed by western blot with IFT88 antibody (13967-1-AP) at a dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Loading control antibodies
GAPDH Antibody
Catalog no.: 60004-1-Ig

GAPDH is commonly used as a protein loading control in western blot due to its consistently high expression in most cell types. This enzyme participates in several cellular events such as glycolysis, DNA repair, and apoptosis.

Proteintech monoclonal GAPDH antibodies are raised against a whole-protein antigen of human origin and have over 2,670 citations.

mouse monoclonal GAPDH antibody WB analysis of HeLa cells
Beta Actin Antibody (KD/KO validated)
Catalog no.: 66009-1-Ig 

Beta-actin is usually used as a loading control due to its broad and consistent expression across all eukaryotic cell types and the fact that expression levels of this protein are not affected by most experimental treatments.

66009-1-Ig has been cited over 1,135 publications and has wide species reactivity.

WB analysis of Jurkat cells using using beta actin antibody (66009-1-Ig)

Proteintech control antibodies are just $99 each (150 in Europe) for a 150ul size vial.

Ready-to-use protein ladders
Prestained Protein Ladder (free samples available) Broad Range Prestained Protein Ladder
Catalog no.: PL00001 Catalog no.: PL00002
Molecular weight coverage: 10-180 kDa Molecular weight coverage: 3-245 kDa
No. of markers: 10 prestained proteins No. of markers: 13 prestained proteins
Proteintech's new prestained protein marker (PL00001) is a three-color protein ladder with 10 markers covering a wide range of molecular weights from 10 - 180 kDa Proteintech's new broad range protein marker (PL00002) is a three-color protein standard with 13 markers covering a wide range of molecular weights from 3 - 245 kDa
Resolution of the prestained protein ladder in a 10-20% Tris-glycine gel (SDS-PAGE).  Resolution of the prestained protein ladder in a 4-12% Bis-Tris gel (SDS-PAGE)

 

References

1. Primary cilia regulate mTORC1 activity and cell size through Lkb1, (PMID: 20972424).

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Posted:
28 August, 2020

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