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IFT88 Antibody

KD/KO Validated

KD/KO Validated

Antibody Catalog number Type Applications  
IFT88 13967-1-AP Rabbit Poly ELISA, IF, IHC, IP, WB 121 Publications

Among its many functions, IFT88 helps mediate intraflagellar transport, which is essential for the assembly and maintenance of cilia and flagella in many organisms. The Proteintech polyclonal IFT88 antibody has appeared in over 100 publications to date.

One of these is related to Kif3 protein, and its role in mammalian Hedgehog (Hh) signaling, where researchers used anti‑IFT88 antibody as a marker for cilium retraction along with acetylated tubulin in IF staining.¹

Immunofluorescent images of MDCK cells stained with IFT88 rabbit pAb (13967-1-AP) and acetylated tubulin mouse mAb (66200-1-Ig) at a dilution of 1:50, further stained with Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) for 13967-1-AP, and Alexa Fluor 488-conjugated AffiniPure Goat anti-Mouse IgG (H+L) for 66200-1-Ig

HEK-293 cells were subjected to SDS PAGE followed by western blot with IFT88 antibody (13967-1-AP) at a dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Free prestained protein ladder (PL00001) samples available
Regular Range Protein Molecular Weight Marker Broad Range Protein Molecular Weight Marker
Catalog no.: PL00001 Catalog no.: PL00002
Molecular weight coverage: 10-180 kDa Molecular weight coverage: 3-245 kDa
No. of markers: 10 prestained proteins No. of markers: 13 prestained proteins
Proteintech's new prestained protein marker (PL00001) is a three-color protein ladder with 10 markers covering a wide range of molecular weights from 10 - 180 kDa Proteintech's new broad range protein marker (PL00002) is a three-color protein standard with 13 markers covering a wide range of molecular weights from 3 - 245 kDa
Resolution of the prestained protein ladder in a 10-20% Tris-glycine gel (SDS-PAGE).  Resolution of the prestained protein ladder in a 4-12% Bis-Tris gel (SDS-PAGE)

References

1. Primary cilia regulate mTORC1 activity and cell size through Lkb1, (PMID: 20972424).

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Posted:
27 March, 2020

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