Western Blot

Proteintech and ChromoTek offer solutions in helping you to achieve publication worthy results in a fast and reliable manner.

Primary Antibodies

Polyclonal Antibodies

Recognize multiple epitopes on the protein of interest. Binds with very high affinity. Good for low abundant targets, rare species and mutated proteins.

Proteintech polyclonals are raised in rabbit.

Monoclonal Antibodies

Recognize a single epitope on the protein of interest. Low background, highly specific. Good for mid-high abundant targets.

Proteintech monoclonal antibodies are raised in mouse.

Recombinant Antibodies

Genetically defined monoclonals, recombinantly produced. Unrivalled reproducibility. Good for long-term, high-level projects. ​

Proteintech recombinant antibodies are raised in rabbit.

Tag/Control Antibodies

Our top cited antibodies for WB loading controls and popular expression tags.

Top Cited Antibodies

Secondary Antibodies

When choosing a secondary antibody for WB, be mindful of:

  1. Host of your primary antibody, E.g. rabbit; and
  2. Your signal detection system

Popular and highly cited secondary antibodies for WB

3,647 Publications

Affinipure Goat Anti-Rabbit IgG (H+L)

Conjugate: HRP
Applications: ELISA, WB
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2,690 Publications

Affinipure Goat Anti-Mouse IgG (H+L)

Conjugate: HRP
Applications: ELISA, WB
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45 Publications

Affinipure Rabbit Anti-Goat IgG (H+L)

Conjugate: HRP
Applications: ELISA, WB
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33 Publications


Conjugate: HRP
Applications: ELISA, WB
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Nano-Secondaries have certain advantages over the conventional secondaries.

  • Pre-incubation or parallel incubation of Nano-Secondaries with primary antibodies, i.e., one-step incubation, reduce incubation times and enhance multiplexing capabilities.
  • The recombinant process of developing Nano-Secondaries ensures high lot to lot consistency.
  • Smaller size of Nano-Secondaries has been proven to enhance image resolution in Super resolution Microscopy.

Multiplexed immunostaining of HeLa cells with two alpaca anti-mouse Nano-Secondaries and one anti-rabbit Nano-Secondary. Green: mouse IgG1 anti-COX4 + alpaca anti-mouse IgG1 VHH Alexa Fluor 488. Magenta: mouse IgG2b anti-Tubulin + alpaca anti-mouse IgG2b VHH Alexa Fluor 647. Yellow: rabbit anti-Lamin + alpaca anti-rabbit IgG VHH Alexa Fluor 568. Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Chemiluminescent Substrates

SignalBright Enhanced Chemiluminescent substrates - luminol-based, chemiluminescent substrate for HRP (horseradish peroxidase) which provides a much higher sensitivity over traditional ECL reagents. We have three reagents available, enabling the detection of proteins low to high femtogram levels.

  SignalBright Pro SignalBright Max
Sensitivity Mid/high to high femtogram Low to mid femtogram
Relative sensitivity to SignalBright Pro 1x 10x
Stable signal duration >12 hours >12 hours
Equivalent SuperSignalTM West Dura SuperSignalTM West Femto
Primary antibody concentration 0.02 - 1 µg/ml 0.01 - 0.2 µg/ml
Secondary antibody concentration 4 - 20 ng/ml 1 - 10 ng/ml

Proteintech’s SignalBright Pro (PK10011) and SignalBright Max (PK10013) Chemiluminescent Substrats have different levels of sensitivity (see above table). In particular, our SignalBrights have comparable performance to top competitor products with highly sensitive detection, minimal optimization required, and long, stable signal duration.

signalbright antibody dilutions Figure 2a: Comparison of SignalBright Pro (PK10011) and SignalBright Max (PK10013) against leading competitor ECL. Serial dilutions of RUVBL primary antibody (10210-2-AP). Primary: Proteintech RUVBL1 (10210-2-AP) at various dilutions (see image). Secondary: Proteintech HRP-conjugated Affinipure Goat Anti-Rabbit IgG (SA00001-2); 1:6,000 Exposure time: 1 min

signalbright protein concentrations Figure 2b: Comparison of SignalBright Pro (PK10011) and SignalBright Max (PK10013) against leading competitor ECL. Serial dilutions of HEK-293 cell lysates. Primary: Proteintech RUVBL1 (10210-2-AP); 1:8,000 Secondary: Proteintech HRP-conjugated Affinipure Goat Anti-Rabbit IgG (SA00001-2); 1:6,000. Exposure time: 1 min

Primary Ladders

Ready-to-use protein standards in three colours. Standard PL00001 and broad range PL00002 markers available. The perfect companion to every WB. Colorimetric detection. Also visible in at red and far-red fluorescent wavelengths.

1 Publication 70 Reviews

Prestained protein marker

Molecular Weight: 10-180 kDa
Number of markers: 10
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3 Reviews

Broad range prestained protein marker

Molecular Weight: 3-245 kDa
Number of markers: 13
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Technical Support for Western Blot

The western blot is an incredibly common application. However, generating good WB data can be hard due to the many facets of a WB workflow that can interfere with results.

Our technical team have put together an excellent guide to Western blotting, describing the overall protocol and a comprehensive trouble shooting section. Download here.