Cardiovascular Research

Proteintech offers a wide range of markers and related antibodies for cardiovascular research

Proteintech offers a wide range of markers and related antibodies for cardiovascular research

Introduction to cardiovascular disease

Age-Associated Changes In Cardiovascular Tissues

mTOR in Cardiac Physiology and Disease

High-Sensitive Cardiac Troponin T

CD31 (PECAM-1): A Multi-Functional Molecule in Vascular Biology

Myosin light chain 2 – Atrial (MYL7/MLC2a) & Ventricular (MYL2/MLC-2v) Cardiomyocytes

MYL7


Introduction

The cardiovascular system is a general term for the heart and blood vessels. Coronary heart disease, cerebrovascular disease, peripheral arterial disease, rheumatic heart disease, congenital heart disease, or deep vein thrombosis and pulmonary embolism are only a few examples of cardiovascular diseases (CVDs). Since CVDs remain a consistently increasing cause of death worldwide, scientists are prioritizing the identification and understanding of the mechanisms of cellular and molecular basics related to CVDs. In 2012, WHO highlighted that more than 30% of our population die because of CVDs (with the majority due to coronary heart disease and stroke).

Age-Associated Changes In Cardiovascular Tissues

Age-associated changes in cardiovascular system are leading to deregulation of molecular pathways and pathological alterations (e.g., hypertrophy, altered left ventricular (LV) diastolic function, abnormalities in arterial stiffness or impaired endothelial function). That reflects in significant increase of CVD including atherosclerosis, hypertension or stroke. Inhibition of the mammalian target of rapamycin (mTOR) presents an evidence for being cardioprotective during aging and cardiac stress.

Blog post:
Hidden Secrets Of The Heart

Focus Antibodies

mTOR in Cardiac Physiology and Disease
Catalog number: 20657-1-AP Type: Rabbit Polyclonal
Publications: 15 Applications: ELISA, IF, IHC, IP, WB

mTOR (Mammalian or Mechanistic Target of Rapamycin) is an atypical serine/threonine kinase that exerts its main cellular functions interacting with specific protein adaptors to form two different complexes: mTORC1 and mTORC2.

The mTOR pathway is a key player in the cardiovascular regulation system. It appears crucial for the maintenance of cardiac structure and function in the postnatal period, adulthood, and also in the development of cardiac hypertrophy. mTOR is also deeply involved in the regulation of cardiac metabolism.

Moreover, the mTOR pathway that partners with Wnt and growth factor signaling are vital for endothelial and cardiomyocyte growth. From the other side, chronic mTOR activation appears to enhance the cardiac aging process.

IF analysis of of HeLa cells labeling mTOR with 20657-1-AP Proteintech antibody at a dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

IHC of paraffin-embedded human testis sample using mTOR antibody (20657-1-AP) at a dilution of 1:50; under 10x.


High-Sensitive Cardiac Troponin T
Catalog number: 15513-1-AP Type: Rabbit Polyclonal
Publications: 4 Applications: ELISA, IHC, WB
The troponin complex regulates the contraction of striated muscles and consists of three subunits (troponin C, troponin T, and troponin I). Cardiac troponin T (cTnT) is a 37 kDa protein that binds to tropomyosin, thereby attaching the troponin complex to the thin filament. cTnT is a cardiac regulatory protein that controls the calcium-mediated interaction between actin and myosin.

Defects in cTnT are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2), cardiomyopathy dilated type 1D (CMD1D) and cardiomyopathy familial restrictive type 3 (RCM3). Statistically significant differences were also described in cTnT levels between newborns with heart defects and healthy individuals.

IF analysis of (4% PFA) fixed mouse heart tissue using Cardiac Troponin T antibody (15513-1-AP; 1:50, 40x) and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC of paraffin-embedded human heart using TNNT2 antibody (15513-1-AP) at a dilution of 1:50; under 40x.


CD31 (PECAM-1): A Multi-Functional Molecule in Vascular Biology
Catalog number: 11265-1-AP Type: Rabbit Polyclonal
Publications: 15 Applications: ELISA, FC, IF, IHC, IP, WB

CD31 (PECAM-1 – platelet endothelial cell adhesion molecule-1) is an Ig gene superfamily member, composed of 6 extracellular Ig folds, with a molecular weight of 130 kDa. It is differentially glycosylated involving N-linked and O-linked glycosylation sites.

CD31 is expressed on all cells within the vascular compartment, on the surface of the endothelium. It plays a role in cell-cell adhesion, being an efficient signaling molecule. This reflects the diverse roles of CD31 in vascular biology including angiogenesis, platelet function, thrombosis, mechanosensing of endothelial cell response to fluid shear stress, and regulation of multiple stages of leukocyte migration through venular walls.

IHC of paraffin-embedded human endometrial cancer sample using CD31 antibody (11265-1-AP) at a dilution of 1:50; under 40x.

 IHC of paraffin-embedded human hepatocirrhosis sample using CD31 antibody (11265-1-AP) at a dilution of 1:50; under 40x.


Myosin light chain 2 – Atrial (MYL7/MLC2a) & Ventricular (MYL2/MLC-2v) Cardiomyocytes
Catalog number: 10906-1-AP Type: Rabbit Polyclonal
Publications: 44 Applications: ELISA, FC, IF, IHC, IP, WB

MYL2/MLC-2v and MYL7/MLC2a are commonly known as two major isoforms of myosin light chain 2, which are essential for heart development.

MYL2/MLC-2v and MYL7/MLC2a are expressed in the heart in a defined manner. The ventricular myosin light chain-2 isoform (MYL2/MLC-2v) is restricted to the ventricular segment of the heart and is related to the ventricles throughout the developing and adult human heart. In contrast, the atrial myosin light chain-2 (MYL7/MLC-2a) is expressed in the presumptive ventricle prior to MYL2/MLC-2v. Its ventricular expression is subsequently down-regulated, instead, with abundant expression in the atrium postnatally.

The expression pattern of MYL2/MLC-2v and MYL7/MLC2a is considered as the specific marker for mature ventricle and atrial cardiomyocytes, commonly used for in vitro development of induced pluripotent stem cell-derived cardiomyocytes.

IHC results of paraffin-embedded human heart tissue using MYL2 antibody (10906-1-AP; 1:200, 40x).

IF analysis of (4% PFA) fixed mouse heart tissue using MYL2 antibody (10906-1-AP; 1:500, 40x) and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).


MYL7
Catalog number: 17283-1-AP Type: Rabbit Polyclonal
Publications: 1 Applications: ELISA, IF, IHC, IP, WB
IHC results of paraffin-embedded human heart tissue using MYL7 antibody (17283-1-AP; 1:100, 40x) IF analysis of (4% PFA) fixed mouse heart tissue using MYL7 antibody (17283-1-AP; 1:50, 40x) and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
More Related Antibodies

The full list can be found here.

Antibody Name Catalog number Type Applications Publications
ACTA2/smooth muscle actin 14395-1-AP Rabbit Poly ELISA, FC, IF, IHC, IP, WB 57
AKT1 10176-2-AP Rabbit Poly ELISA, FC, IF, IHC, IP, WB 56
ALDH2 15310-1-AP Rabbit Poly ELISA, IF, IHC, IP, WB 7
APEX1 10203-1-AP Rabbit Poly ELISA, IF, IHC, IP, WB 5
Catalase 21260-1-AP Rabbit Poly ELISA, IF, IHC, WB 10
CD31 11265-1-AP Rabbit Poly ELISA, FC, IF, IHC, IP, WB 14
HEXIM1 15676-1-AP Rabbit Poly CoIP, ELISA, IF, IHC, IP, WB 7
Hexokinase 2 22029-1-AP Rabbit Poly ELISA, IHC, IP, WB 7
HEY2 10597-1-AP Rabbit Poly ELISA, IF, IHC, IP, WB 15
Icam1 10020-1-AP Rabbit Poly ELISA, FC, IF, IHC, WB 15
ICAM-1 10831-1-AP Rabbit Poly ELISA, FC, IHC, IP, WB 13
MEF2C 10056-1-AP Rabbit Poly ELISA, IF, IHC, IP, WB 15
Mitofilin 10179-1-AP Rabbit Poly ELISA, FC, IF, IHC, IP, WB 14
MTOR 20657-1-AP Rabbit Poly ELISA, IF, IHC, IP, WB 13
Myosin Light Chain Antibody 2 10906-1-AP Rabbit Poly ELISA, FC, IF, IHC, IP, WB 44
NRF2, NFE2L2 16396-1-AP Rabbit Poly ELISA, FC, IF, IHC, IP, WB 22
PROX1 51043-1-AP Rabbit Poly ChIP, CoIP, ELISA, IF, IHC, WB 8
Sirt 1 13161-1-AP Rabbit Poly ELISA, IF, IHC, IP, WB 25
SNAI1 13099-1-AP Rabbit Poly ELISA, IHC, IP, WB 17
STAT3 10253-2-AP Rabbit Poly ChIP, ELISA, FC, IF, IHC, IP, WB 13
SUCLA2 12627-1-AP Rabbit Poly ELISA, IF, IHC, IP, WB 8
TFAM 19998-1-AP Rabbit Poly ELISA, IHC, WB 8
transgelin/ SM22 10493-1-AP Rabbit Poly ELISA, FC, IF, IHC, WB 22
TWIST1 18125-1-AP Rabbit Poly ELISA, WB 9
VCAM1 11444-1-AP Rabbit Poly ELISA, FC, IF, IHC, WB 10
Download the full catalog of antibodies for cardiovascular research here

 

Featured Products

Posted:
30 May, 2017

Share:


Back
to top