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Antibody Labeling Kits

FlexAble is a novel antibody labeling kit that uses an affinity linker to conjugate fluorochromes, enzymes, and molecules in any buffer condition - now with increased brightness to maximize your multiplex results.

FlexAble Antibody Labeling Kits revolutionized antibody labeling by allowing you to add dyes and molecules to any antibody, any time. We’ve improved our original FlexAble to allow for more dyes per antibody and a better, brighter signal with FlexAble 2.0 New.

  • Compatible with primary antibodies from any supplier
  • Label as little as 0.5ug. No buffer exchange needed.
  • Works with any antibody concentration and formulation
  • Enable same species multiplexing
  • Fast and easy 2-step protocol – Ready to use in 10 minutes
  • No additional equipment required
Watch how FlexAble works New Try our FlexAble calculator View all FlexAble antibody labeling kits

Research Area

Immunofluorescence Imaging of Live and Fixed Pancreatic Tissue Slices Using FlexAble 2.0 Antibody Labeling

Testimonial

Scientist Stories: Léa Pechtimaldjian on Advancing 3D Vascular Imaging with FlexAble Technology

Application Note

The FlexAble way to improve your immunofluorescence with IBEX

Whitepaper

Efficient Hybridoma Screening with FlexAble Antibody Labeling Kits

Available FlexAble 2.0 Antibody Labeling Kits

Sizes: 10 rxn, 50 rxn, 200 rxn sizes.

Original FlexAble Kits are still available upon request here.

Species CoraLite® Plus Other conjugates
Rabbit IgG 405 488 555 594 647 750 FITC Plus HRP Biotin
Mouse IgG1 405 488 555 594 647 750 FITC Plus HRP Biotin
Mouse IgG2a 405 488 555 594 647 750 FITC Plus HRP Biotin
Mouse IgG2b 405 488 555 594 647 750 FITC Plus HRP Biotin
Human IgG 405 488 555 594 647 750 FITC Plus HRP Biotin
Rat Kappa Light Chain 488 555 647 FITC Plus Biotin
View protocol and technical FAQs View protocol and technical FAQs What are CoraLite® Plus conjugates?

CoraLite® and CoraLite® Plus dyes have equivalent brightness to the Alexa Fluor® dyes. Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Comparison of FlexAble labeled antibodies to secondary antibody detection

Comparison of different mouse and rabbit antibodies from variety of IgG subclasses were labeled with the original FlexAble kits, new FlexAble 2.0, or as a primary and secondary antibody combination.

Eight different mouse and rabbit antibodies of different IgG subclasses were labeled with either Primary IgG + FlexAble 2.0, or with a primary and secondary IgG combination. Green: FlexAble 2.0 CoraLite Plus 488; Magenta: FlexAble 2.0 CoraLite Plus 647 or secondary antibodies labeled with the respective dyes. Mean fluorescent intensities are given to compare relative signals of each approach.

Antibody labeling with FlexAble is achieved by using a high-affinity, non-covalent FlexLinker. The FlexLinker binds tightly to the antibody within minutes and once bound does not release from the antibody. Unbound FlexLinker is neutralized by adding a FlexQuencher.

The labeling reaction using FlexAble yields a homogenous, well-defined antibody that is fully functional. Neither aggregation nor a loss of antibody is observed during the labeling.

Antibody labeling with Proteintech's FlexAble kit is achieved by using a high affinity non-covalent linker that binds tightly to the antibody within minutes and remains attached indefinitely.

FlexAble and FlexAble 2.0 Labeling kits can be used to label:

  • Primary antibodies from any supplier
  • Antibodies of any concentration
  • Antibodies in any buffer formulation

FlexAble Antibody Labeling Kits have been thoroughly validated with primary antibodies from different suppliers. Primary antibodies with low concentration, antibodies stored in 50% glycerol, and antibodies supplemented with carriers like BSA are compatible for use with the FlexAble kits.

FlexAble and FlexAble 2.0 Antibody Labeling Kits work with any antibody formulation. Labeling with the high-affinity FlexLinker is not affected by stabilizers like BSA or glycerol, amine-containing buffers, or preservatives.

Antibody buffer formulation

FlexAble Antibody Labeling Kits work with any antibody formulation without the need to purify or buffer-exchange prior to labeling. This saves time and money and also avoids the loss of antibody that can happen during those processes. Since FlexAble utilizes a high-affinity FlexLinker molecule that binds site-specifically to an antibody, the binding reaction is not affected by carriers like BSA or glycerol, amine-containing buffers like Tris, or preservatives like sodium azide.

Comparison of CD3 antibody stored in various glycerol concentration prior to labeling with FlexAble antibody labeling kit test

Anti-CD3 antibody, previously stored in the indicated concentration of glycerol, was efficiently labeled with FlexAble Antibody Labeling Kit regardless of the glycerol concentration during labeling. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with 0.5 µg anti-human CD3 antibody (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023) . Cells were gated for live singlet lymphocytes.

Antibody Concentration

A highly concentrated antibody can be used without pre-dilution, or an antibody of low concentration can be used without further concentrating it. FlexAble is especially suitable for low-concentration antibodies due to the high affinity of the FlexLinker. Antibody concentrations as low as 0.06 mg/mL have been efficiently labeled with FlexAble.

Labeling antibodies with FlexAble is fast and easy due to a simple 2-step protocol. The procedure only requires about 30 seconds of hands-on time and only a pipette is needed. Mix your primary antibody, FlexLinker, and FlexBuffer together and incubate for 5 minutes. Add FlexQuencher to neutralize unbound FlexLinker and incubate again for 5 minutes. The antibody is ready for use in the time it takes to label your tubes. The same protocol is suitable for both FlexAble and FlexAble 2.0 Antibody Labeling Kits.

Step-by-step workflow of Proteintech's FlexAble antibody labeling kit shows 30 seconds hands-on time and ready-to-use in less than 10 minutes

FlexAble Antibody Labeling Kits simplify multiplexing in immunofluorescence, western blotting, and flow cytometry. Save hands-on and incubation time by completing labeling of all your antibodies in 10 minutes before your experiment, followed by multiplex staining with all labeled antibodies at the same time. If primary antibodies of different species are used, they can even be labeled simultaneously in the same tube with FlexAble.

Primary antibodies of different species can be labeled simultaneously in the same tube with FlexAble antibody labeling kits.

Antibodies labeled with FlexAble and FlexAble 2.0 Antibody Labeling Kits can be used alone or in multiplex experiments with:

  • Other antibodies labeled with FlexAble Antibody Labeling Kits
  • Commercially available pre-conjugated primary antibodies
  • Other primary and secondary antibody combinations

Immunofluorescence of rat brain tissue stained using NueN antibody, TUBB3 antibody and GFAP antibody

Immunofluorescence of rat brain tissue: FFPE rat brain tissue sections were stained with anti-NeuN antibody (66836-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, magenta), anti-TUBB3 antibody 66375-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, orange), and CoraLite®488-conjugated GFAP antibody (CL488-60190, green).

FlexAble enables same species multiplexing

Same species multiplexing usually requires complex protocols that include primaries from different species/isotypes combined with highly specific and cross-adsorbed secondary antibodies. An alternative is pre-conjugated antibodies; however, not every antibody may be available in a pre-conjugated version.

FlexAble provides the easiest way to multiplex antibodies from the same species and isotype

FlexAble provides the easiest way to multiplex antibodies from the same species and isotype. FlexLinker binds to the primary antibody with high affinity and specificity and does not release from the antibody once bound. Therefore, antibodies from the same species or isotype can be incubated with the sample at the same time after being labeled with FlexAble. No leaking or cross-binding to other antibody isotypes has been observed.

immunofluorescence staining of HeLa cells with TDP43 antibody,] and Lamin B1 antibody

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TDP43 antibody (10782-2-AP) labeled with FlexAble CoraLite® 488 Kit (KFA001, green), anti-Tom20 antibody (11802-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, red), and anti-Lamin B1 antibody (12987-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, cyan). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Proteintech's CoraLite Plus excitation and emission

CoraLite Plus spectra

CoraLite® and CoraLite® Plus dyes are bright fluorescent dyes from Proteintech that deliver high photostability and minimal fluorescent bleed through, making it easy to combine two or more dyes in an experiment.

Our FlexAble and FlexAble 2.0 Kits are conjugated to multiple CoraLite® Plus dyes to offer more flexibility in multiple labeling experiments.

Learn more about CoraLite® and CoraLite® Plus dyes