Organoid Research Solutions

Organoids are more physiologically relevant models than traditional 2D cultures, transforming the outcomes of basic research, preclinical model development, and clinical applications. Our comprehensive reagent offering of high-quality growth factors, antibodies, ELISA kits, and more is carefully curated to enhance organoid workflow experience.

Validation in organoid models

Well-validated reagents ensure experimental consistency and improve the physiological relevance of organoid-based models in drug discovery and disease modeling.

GMP grade for clinical manufacturing

Comprehensive portfolio of GMP-grade reagents streamlines workflow optimization and accelerates the development of organoid-based therapeutics and personalized medicine.

Organoid workflow optimization support

Expert guidance to fine tune the reagent concentrations, exposure timing, and handling technique to accelerate the path to an optimized workflow development.

Comparison of Humankine (HEK 293) BDNF and E.coli derived BDNF recombinant protein

Neuronal differentiation of SHSY5Y cells using BDNF and retinoic acid showing higher neuronal differentiation using HumanKine BDNF in comparison to E. coli derived BDNF.

HumanKine

HumanKine is a line of recombinant human growth factors produced in HEK293 human cells, offering native human post-translational modifications and correct protein folding that enhance biological activity, facilitating improved cell differentiation. HumanKine is animal component free (ACF), which eliminates the risk of contamination from animal-derived pathogens and reduces variability caused by undefined serum components, ensuring higher consistency and reproducibility in experiments. It is also the largest selection of GMP grade growth factors, which further supports translating various organoids from research to clinical application.

Antibodies

Proteintech offers a comprehensive portfolio of conjugated antibodies, including the industry-leading CoraLite® and CoraLite® Plus dye series. CoraLite dyes are engineered for superior brightness, photostability, and minimal spectral overlap, making them ideal for high-content imaging and multiplex imaging of complex organoids. This expansive offering can streamline organoid characterization and gain deeper insights into disease modeling, drug screening, and regenerative medicine.

CoraLite Plus 488 IF validation comparison with competitor's 488

Immunofluorescence of a nuclear protein with CoraLite Plus-488 antibody in comparison to a similar product from a leading competitor demonstrating higher signal to background ratio for CoraLite Plus.

Proteintech ELISA Cystatin C comparison with competitor's

Detection of Cystatin C using ELISA kits demonstrates 10X higher sensitivity for AuthentikineELISA in comparison to competitor ELISA.

ELISA Kits

Proteintech ELISA kits offer superior performance by deriving antibodies and standards from the same immunogen, ensuring unmatched accuracy and consistency in quantification. This design minimizes cross-reactivity, which is especially important when analyzing complex samples like the organoid secretome. AuthentiKine kits are validated with recombinant proteins expressed in human cells, making them more biologically relevant and reflective of native protein structure and function. Their high sensitivity and lot-to-lot consistency make them ideal for detecting subtle changes in cytokine and growth factor levels in organoid cultures.

Products for organoid differentiation and characterization

Organoid Type Growth Factor Characterization Marker
Cerebral Organoids EGF PAX6
FGF2 SOX2
BDNF TBR1
NT3 TUJ1
   MAP2
GFAP
Retinal Organoids IGF-1 PAX6
DKK1 CRX
Noggin NRL
FGF2 VSX2
  Recoverin
Lung Organoids Wnt3a NKX2.1
EGF SOX2
Noggin SOX9
FGF10 SFTPC
BMP4 FOXJ1
KGF (FGF7)  
Intestinal Organoids EGF CDX2
Noggin LGR5
R-spondin1 MUC2
Wnt3a Villin
   Lysozyme
Chromogranin A
Gastric Organoids EGF SOX2
FGF10 PDX1
Noggin MUC5AC
Wnt3a GATA4
Liver Organoids HGF AFP
EGF ALB
FGF10 HNF4α
Oncostatin M CK18
Wnt3a CYP3A4
Pancreatic Organoids EGF PDX1
Noggin NKX6.1
R-spondin1 INS
Wnt3a GCG
Betacellulin    
KGF (FGF7)
FGF10
Kidney Organoids Activin A WT1
GDNF PAX2
FGF9 E-Cadherin
  NPHS1
Cardiac Organoids FGF2 cTnI
BMP4 NKX2.5
Activin A cTnT
VEGF MYL2
  ACTN2
Thymic Organoids BMP4 FOXN1
KGF (FGF7) KRT5
FGF10 KRT8
   AIRE
DLL4
Esophageal Organoid Wnt3a P63
R-spondin1 SOX2
Noggin KRT5
FGF10 KRT13
  NKX2.1
Prostate Organoid Wnt3a AR
R-spondin1 NKX3.1
Noggin KRT8
FGF10 KRT5
EGF PSA
 

Validation Data Gallery

Technical Resources