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A Practical Guide for Dendritic Cell Identification by Flow Cytometry
Written by: Sepideh Jahangiri, PhD in Experimental Medicine at Université de Montréal
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Streamlining Multiplex Imaging in Expanded Tissues with FlexAble 2.0 Antibody Labeling Kits and Magnify™ Expansion Microscopy
Authors: Aleksandra Klimas, Tina Ryu, and Yongxin (Leon) Zhao, Magnify Biosciences, Inc.
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A Practical Step-by-Step Guide to Subcellular Fractionation in Mammalian Cells
By Alexandra-Teodora Szabo, Postgraduate Researcher in Mitochondrial Biology at University of Tübingen
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Cardiac Organoids in Research
Written By Anna Katharina Schrattel – University Hospital of Heidelberg, University of Heidelberg
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Tertiary Lymphoid Structures: What They Are and Why They Matter in Immuno-oncology
Written by Sarah Wilkey, PhD Student at the University of Queensland, Australia
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PhD Data Management Guide: How to Organize, Label, and Store Research Data Effectively
Written by Sara Bitar, PhD researcher at Johannes Gutenberg University of Mainz, Germany
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Guardians of the gut: Gamma-delta T cells and their emerging role in the gut-immune-brain axis
Written by Rogério Rocha de Castro, PhD candidate in Medical Neuroscience at Radboud University Medical Center
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A Guide to Markers for Oligodendrocyte Identification
Written by Tessa Dallo, PhD student at Florida Atlantic University
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Scientist Stories: Dr Rebecca Saleeb on Multiplex Imaging Possibilities with Secondary Nanobody Technology
How Dr Saleeb is using Proteintech antibodies and nanobodies to unlock new flexibility in multiplexed immunofluorescence for ALS
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Beyond The Lab: Real Scientist Stories
Written by Alexandra-Teodora Szabo, PhD student in Mitochondrial Biogenesis at University of Tübingen
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You Shall Not Pass! - The Blood-Brain Barrier
Beatriz Achón Buil, PhD candidate in Neuroscience, Institute for Regenerative Medicine (University of Zürich)
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Microglia – An Update on How to Define this Multifaceted Cell Population
Written by Maria-Luisa Wiesinger; PhD student in pediatric low-grade glioma research at the German Cancer Research Center, Heidelberg
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Scientist Stories: Revealing BAG3’s Role in Tau Clearance with Proteintech Antibodies
Written by Hayley Wnuk, PhD, Post-Doctoral Research Associate at the University of Rochester Medical Center
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Scientist Stories: Charlotte Scholtes on Unlocking ER–Mitochondria Contacts with Proteintech Antibodies
How Charlotte Scholtes, PhD used Proteintech antibodies to accelerate research into ER–mitochondria contacts and metabolic regulation.
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The power of neutrophils: How to identify the immune system’s first responders
By Maria Alejandra Feliz Norberto, M.S., PhD Candidate at Albert Einstein College of Medicine
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AI in Neuroscience: Science-Fiction or Reality?
Written by Daniel Doyon, PhD student at Karolinska Institutet in Stockholm
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Tips on your coding journey as a PhD student
Written by María Bueno Álvez, PhD candidate at Science for Life Laboratory
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Scientist Stories: Fabienne Bejjani, PhD on Improving Research Efficiency with Proteintech Antibodies
Exploring how high-quality, fast-delivery antibodies from Proteintech enhanced experimental accuracy and reproducibility in molecular biology research.
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Scientist Stories: Léa Pechtimaldjian on Advancing 3D Vascular Imaging with FlexAble Technology
How Léa Pechtimaldjian used Proteintech antibodies and FlexAble technology to accelerate 3D vascular imaging.
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The Gut Microbiome and Depression: Current Insights and Future Directions
Written by Hasnain Methiwala, PhD Student at the University of Minnesota Medical School
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Immunofluorescence Imaging of Live and Fixed Pancreatic Tissue Slices Using FlexAble 2.0 Antibody Labeling
Written by Vijay Sai Josyula, Ph.D. Candidate at Uppsala University
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Research Grant Stories: Dr Elisa Damo on Mapping Antibody Distribution in Pain Models
How Proteintech Research Grants allow researchers to advance their projects. #InTheLabWithProteintech
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Endothelial Cells: Gatekeepers in HIV Disease Progression
Written by Bryant Avalos, PhD, Postdoctoral Researcher at University of California, San Diego
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How Organ-Chip Technology is Redefining Human Physiology and Disease Modelling
Written by Catrin Bevan, PhD Student at Queen Mary University of London.
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Hematopoietic Stem and Progenitor Cells (HSPCs): What They Are and How to Identify Them
Written by Maria Alejandra Feliz Norberto, M.S., PhD Candidate at Albert Einstein College of Medicine
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What is Optogenetics? How Scientists are Using Light to Understand the Brain
Written by Anastasiia Caangay, PhD candidate at the University of Alabama-Birmingham
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Imposter Syndrome in Scientific Research: The Hidden Epidemic
Written by Hannah Joanne Lee, PhD Candidate at Queen Mary University of London
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How to Write a Strong Statement of Purpose for a PhD Program
Written by Breanna Lam, PhD Candidate at Massachusetts Institute of Technology
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Get the Most from Your iPSCs: Improved Cardiomyocyte Differentiation
Written by Jamuna Karanankattil Sukumaran, Senior Scientist at Proteintech
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Targeting Purinergic Signaling: A New Frontier in Cancer Immunotherapy
Written by Sepideh Jahangiri, PhD, University of Montreal
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Markers for Astrocyte Identification
Written by Haddie DeHart, PhD student at Indiana University School of Medicine
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Ferritinophagy: Understanding Its Roles in Iron Homeostasis and Disease
Written by Elizabeth Byers, PhD Candidate at The Pennsylvania State University
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The Heart of the Matter: Voltage-Gated Calcium Channels in Cardiovascular Disease
Written by Hannah Joanne Lee, PhD Candidate at Queen Mary University of London
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Commanding Endosomal Sorting: The Role of the Commander Complex in Protein Trafficking
Written by Kincaid Ingram; PhD researcher focusing on endosomal biology in health and disease at the University of Bristol, UK
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The importance of epitope selection in experimental design
Discover how pinpointing the right antibody binding site can transform the reliability and impact of your research, diagnostics, and therapeutic strategies.
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Every protein tells a story
Unveiling the story of proteins—One epitope at a time
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Identifying Cortical Cell Types Through Immunofluorescence and Electrophysiology
Written by Airi Watanabe, Ph.D. Candidate at McGill University
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Everything is an Opportunity: A science communication interview with Professor Manasi Nandi
Written by Amanda Bowman, PhD Candidate at King’s College of London
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Clopidogrel Dual Antiplatelet Therapy and Novel Treatments for Atherothrombosis
Written by Shaden Salimi Graduate Student at McGill University
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A Guide to Studying Wild-Type and Mutant Proteins
Written by Suparba Roy, PhD Candidate at Université Laval
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Antibody Validation for ELISA: Ensuring Accurate and Reliable Results
Written by Nerea Gómez de San José, PhD in protein biomarker research.
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How To Write a Literature Review: A Comprehensive Guide
Written by Dr. Reshma Patil
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Immune Checkpoint Inhibitor Therapy: Revolutionizing Cancer Treatment
Written by Sepideh Jahangiri, PhD Candidate at the University of Montreal
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Understanding FGF Signaling: A Key Pathway in Development and Disease
Written by Jaime Fernández Sobaberas, 3rd year PhD student in Biochemistry at Heidelberg University, Germany.
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The FlexAble way to improve your immunofluorescence with IBEX
Written by Aanandita Kothurkar and Natalia Jaroszynska from UCL Institute of Ophthalmology
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A Guide to Building a Direct Sandwich ELISA
Written by Dr. Reshma Patil
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A Guide to Immunostaining the Cerebellum
Written by Sophia Leung, PhD, Postdoctoral Fellow at McGill University
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Cuproptosis in Health and Disease: Copper’s Double-Edged Sword
Written by Jeyashree Alagarsamy, PhD Candidate at the University of Cincinnati
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Mimicking the Extracellular Matrix Through Electrospinning
Written by Elizabeth Byers, PhD Candidate at The Pennsylvania State University
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Nanobodies in Structural Biology
Written by George Biggin, Structural Biology PhD Student at The University of Manchester
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Understanding Liver Cancer: Causes, diagnostics, and therapeutic avenues
Written by Selin Dayan-Özdurmus, PhD researcher at German Cancer Research Center (DKFZ), Heidelberg
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Protein Phosphorylation; Not just a binary switch.
Written by Joris Frenz; Proteomics and Cancer cell signaling PhD researcher at the German Cancer Research Center, Heidelberg
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Immunofluorescence techniques in zebrafish retina
Written by Natalia Jaroszynska, PhD Candidate at UCL Institute of Ophthalmology
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Navigating Nematodes: Tips for Successful Worm Picking
Written by Levon Tokmakjian, PhD Candidate at University of Toronto
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5 creative ways to stay productive in graduate school
Written by Sophia Leung, PhD from the University of McGill
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What is Macrophage Polarization?
Written by Consuelo Coser, PhD Student at University of Nottingham
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How to transition from academia to industry using LinkedIn
Written by Elena Hoffer, PhD. Co-Founder and CEO of Alma.me.
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Unlocking the Secrets of Aging: The Role of Epigenetics in Cellular Senescence
Written by Shiksha Dutta, PhD student at University of Montreal
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How to create a LinkedIn profile for scientists
Discover how to optimize your LinkedIn profile to promote yourself and your science.
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Guide to Flow Cytometry Panel Building
Written by Dr. Frédéric Duval and Sari Gezzar-Dandashi from the Maisonneuve-Rosemont Hospital Research Center.
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How to choose the right secondary antibody?
How to choose the best secondary antibody for your application.
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PLSCR1: An exciting new defender against COVID-19
Written by Sophie Williams; Biochemistry PhD researcher at the University of Bristol.
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How to Better Study Ubiquitination
Understand the role of Ubiquitin in protein degradation and learn how ChromoTek’s Ubiquitin Trap can help you overcome certain ubiquitin-related research challenges.
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Quartz Crystal Microbalance: Next-generation biosensor?
Understand how the Quartz Crystal Microbalance technique could be the future of biosensing and biomarker detection.
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How to culture mouse primary cerebral microvascular endothelial cells
Learn to how to isolate and culture these integral cells types for blood brain barrier research.
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m5C Modifications in RNA and Cancer
Explore how the epigenetic modification m5C regulates the fate of RNA molecules and cancer prognosis.
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Decoding the Mitochondrial Unfolded Protein Response
Summary of a Nature paper from Goethe University Frankfurt revealing the previously unknown mechanisms of the mitochondrial unfolded protein response.
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Tips and tricks for iPSC culture
Top tips for successful induced pluripotent stem cell and embryonic stem cell culture.
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A Guide to Staining Organelles
Identifying subcellular structures is key for mapping the localization of target proteins. This blog features key markers to identify organelles by immunofluorescence.
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Under the hood of t-SNE – the t-SNE algorithm explained
Walk step-by-step through how t-SNE represents your high-dimensional data in two dimensions.
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8 Tips for Networking at Scientific Conferences
Here are our top tips on how to use conferences to make meaningful connections.
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B cell mitochondria: much more than a powerhouse
Nature Immunology paper from a team the University of Oxford detail how mitochondria are essential for B cell development and lymphomagenesis.
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Biomarkers for neurodegenerative disorders
Relevant fluid biomarkers are required to enable early and accurate diagnosis of neurodegenerative diseases. This blog details current biomarkers and promising future biomarkers.
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Introduction to t-SNE for Flow Cytometry
Understand the pros and cons of t-SNE, an algorithm for visualizing high-dimensional data
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Time management and self-care in research
Tips and tricks to help improve efficiency and personal care for early career researchers.
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Key considerations for raw material selection in cell therapy manufacturing
Learn more about regulatory requirements and best practices in sourcing raw materials for cell based therapy manufacturing
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Scientists Discover Dynein-Driven Motility in Primary Cilia!
Researchers have discovered that the movement of primary cilia on pancreatic cells assists in insulin secretion
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Exposure to microplastics increases risk of liver fibrosis
Written by Ryan Murray, PhD Student at the University of Leeds
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RIP/RIP-seq to study RNA Modifications
Written by Sripriya Raja, PhD Candidate at University of Pittsburgh.
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The Role of Autophagy in Cancer
Written by Dharaniya Sakthivel, PhD student at Baylor College of Medicine.
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Novel organoid model enables study of microglia in health and disease
Scientists develop a new human brain organoid to include immune cells, making it more realistic for physiological and pathological research.
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How to Review a Scientific Paper in 10 Easy Steps
Summary of how to perform an invited review of a paper for publication.
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How to make a scientific conference poster
Our top tips to make an impactful poster at your next conference
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Scientists Validate Biomarker Assay for Parkinson’s Disease!
A clinical study shows that α-synuclein seed amplification assays (SAAs) can reliably identify people with Parkinson’s disease.
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Go with the Flow - Make the most of this powerful technique
Discover all the ways flow cytometry can be used to take your research further
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The Importance of Self-Care While Doing your PhD
by Rachel Surratt, MA, Associate Professional Clinical Counselor at Resiliency and Health Institute, LLC
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Protein tags: Advantages and disadvantages
Understand when it is beneficial to tag a protein and when it is better to stay tag free.
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Spectral Compensation in Flow Cytometry
Excerpt from Mastering Flow Cytometry: Best Practices and Tips webinar given by Dr. Shweta Puntambekar, Ph.D. This webinar excerpt has been edited for clarity.
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How to Navigate Finishing Your Ph.D. Remotely
Written by Sepideh Dadkhah, Ph.D. Candidate at the University of Kentucky
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Mitochondrial respiratory supercomplexes: a focus on COX7A isoforms
COX7A isoforms adapt the cellular energy production machinery to metabolic demands.
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How to write a PhD thesis: 10 top tips
Written by Kathryn Green, Final Year Ph.D. Student at the University of Nottingham.
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The role of ferroptosis in human diseases
Written by Dharaniya Sakthivel, PhD student at Baylor College of Medicine
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ChatGPT and BioGPT as tools for life science research
Written by Benjamin Raven, PhD student researching cellular senescence at the University of Sheffield, UK.
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Flow Cytometry Gating for Beginners
A step-by-step guide to flow cytometry gating for easy analysis and clean, publication-ready data.
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Detecting RNA Methylation by Dot Blotting
A step-by-step guide by Proteintech scientists!
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New avenue for treatment of ALS and frontotemporal dementia? A canonical m6A reader is a potential new target for ALS and frontotemporal dementia
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Introduction to cerebral organoids in research
Understand how brain organoids are made and why they are tipped to revolutionize life science research models.
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Long-Term Effects of COVID-19 on the Gastrointestinal System
Written by Rahul Panchal, Scientist II at Proteintech Group in Chicago, IL.
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Vascular dysfunction in Alzheimer’s Disease
Written by Katy Walsh, PhD student researching vascular dementia at the University of Manchester, UK.
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Women in Science
International Day of Women and Girls in Science
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Tips for detecting phosphoproteins by western blot
Tips and tricks to optimize your western blot for phosphoprotein detection
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Science paper increases understanding of how ALS and frontotemporal dementia pathology interact
Take a closer look at recent Science paper by Shao et al
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International Unit-based Specific Activity Determination for HumanKine® Products
Recombinant protein biological activity can be quantified by international units (IU) based on comparisons to a centralized set of standards
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What is the difference between pyroptosis and apoptosis?
Written by Mehak Passi, PhD Researcher at LMU Munich University
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Reverse Phase Protein Array – a high throughput proteomic tool
Discover the potential of RPPA and its many advantages over other proteomic techniques
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How to culture Bone Marrow Derived Macrophages
Our top tips to successfully extract and culture murine Bone Marrow Derived Macrophages
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Breast Cancer Awareness Month
October is an annual health campaign to promote early detection, reduce risk factors and support patients
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How to start your own business after a PhD: an insider’s perspective
Looking to move from academia to industry? Get advice from Dr. Chris Bullock, CEO and Co-Founder of QV Bioelectronics, on how to become an entrepreneur after finishing your PhD.
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How to Identify Activated Microglia
Proteintech experts share ways to quantify your microglial cell population
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Building research careers in a post-COVID world
Creative strategies used by early-career researchers during the pandemic to establish themselves could launch careers after the pandemic fades.
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How to prepare for a PhD viva - Ten tips for success
Written by Tiago Marcos, PhD, University of Edinburgh.
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7 Tips to successfully culture primary rodent neurons
Proteintech experts provide seven tips for improving the integrity of your primary neuronal cultures.
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Overview of the vascular system and the importance of endothelial cells
Written by Kathryn Green, PhD student at The University of Nottingham.
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Proteintech interview with an early-career researcher: Odetta Antico
Odetta Anitco from the University of Dundee, Scotland, speaks to Proteintech about her scientific career so far, her recent paper in Science Advances, and how to handle setbacks in research.
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Replication Race: Scientists unravel why Omicron spreads faster
Written by Tiago Marcos Ph.D, guest author from the University of Edinburgh.
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Explore cytokeratin dynamics with CoraLite conjugates
Want to learn more about Cytokeratins and their role in disease development? Introducing CoraLite conjugated antibodies against cytokeratins.
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GFP-Booster: GFP Nanobody for better images in immunofluorescence
Higher resolution and better tissue penetration
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How to immunoprecipitate Flag®-tagged proteins
Flag®-tag (or DYKDDDDK-tag) is a commonly used short peptide tag for multiple applications such as immunoprecipitation (IP), protein purification, immunofluorescence, and Western blotting (WB).
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Why is the Fab-Trap™ so Fab-ulous?
ChromoTek’s Fab-Trap™ is an IP reagent based on an antibody Fab-fragment.
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Advantages and limitations of different antibody formats in immunoprecipitation
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Detecting low abundance proteins via Western Blot
Detecting low abundance proteins can be a challenge - here's how to optimize your Western Blot.
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7 Tech Tips For Successful ChIP Experiments
New to ChIP or just want to improve? Our tech tips will help you achieve successful ChIP experiments
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How Nanobodies could advance Sars-CoV diagnostics
Teresa Wagner and Ulrich Rothbauer discuss the development of Nanobodies against SARS-CoV-2 and why Nanobodies are versatile alternatives to classical antibodies in SARS-CoV-2 research.
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The highly adaptive cytoskeleton
The cytoskeleton is a highly important structure present in the cytoplasm of all cells. Learn how this is integral for many cellular functions, and the many ways researchers can study the cytoskeleton in our blog.
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How to conduct a Co-immunoprecipitation (Co-IP)
Co-IP describes the immunoprecipitation of a protein of interest and its interacting partners.
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Co-immunoprecipitation troubleshooting, tips and tricks
Co-immunoprecipitation (Co-IP) describes the isolation of a protein and its binding partners from a cell extract using a Nanobody or antibody that is bound to beads. The protein, that directly interacts with the Nanobody, or antibody beads is called “bait”. The binding partner that is indirectly precipitated is called “prey”.
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Mouse-on-mouse for Immunohistochemistry: tips and tricks
Learn how to mitigate issues with high background staining and perform mouse-on-mouse staining with confidence.
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Want to upgrade your immunofluorescence workflow? Go Direct!
Learn about the advantages of using directly conjugated primary antibodies for IF
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Flag-tag and 3x Flag-tag
Overview about specifications, properties, origin, sequence, size, specifications, etc. of Flag-tag
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How to choose your recombinant proteins, cytokines & growth-factors
Considerations when choosing recombinant proteins for your research applications.
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New ways to study the elusive exosome
Written by Poppy Nathan, Proteintech Field Marketing Representative and PhD Student at the University of Birmingham
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GFP (green fluorescent protein): Properties, origin, specifications, tips
Everything you need to know about GFP: properties and applications
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Ask a Flow Cytometry Expert: Your Questions Answered
Dr. Gareth Howell, Senior Experimental Officer and Flow Core Facility Manager at the University of Manchester answers your questions.
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Tips and tricks for immunoprecipitation of low abundant proteins
For a successful IP, the abundance or expression level of the POI is crucial. If a POI is strongly expressed and highly abundant, then the IP will most likely be straightforward, with no difficulties.
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Navigating a PhD in a Pandemic
Tips and advice for navigating a PhD during a global pandemic, written by guest blogger Francesca Lewns.
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An alternative Nanobody format that combines its special epitope binding properties with the detection options for Fc-domains
A new Swiss army knife for immunofluorescence: Chimeric heavy chain antibodies
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To dye or not to dye? Understanding the different viability dyes available for flow cytometry
Learn about the types of viability dyes available to help you achieve reliable and accurate results in flow cytometry.
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Seizing the day: How to save time on your immunoassay experiments
When time in the lab is limited, learn how to save precious hours on your IP, IF, and western blotting experiments.
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Conjugation of fluorescent dyes to Nanobodies
The conjugation of the binding domains of camelid heavy chain antibodies, also known as VHHs or Nanobodies, to fluorescent dyes is generating versatile tools for fluorescence microscopy applications like immunofluorescence (IF) and super resolution microscopy (SRM):
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Neuropilin-1 joins ACE2 as SARS-CoV-2 gateway
Landmark paper in Science detailing how Neuropilin-1 is required for SARS-CoV-2 host cell entry, using Proteintech and Chromotek antibodies.
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Intracellular Flow Cytometry Staining Protocol
Understand the different approaches to intracellular staining for flow cytometry in this "do-it-yourself" guide to fix and perm.
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Interview with an early-career scientist: Dr Chaitali Mukherjee
Hear from an early-career scientist about her first-author publication in Cell Metabolism and her academic journey
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Using Flow Cytometry with Epigenetics
Experimental considerations for using flow cytometry to investigate epigenetics.
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Exosomes: A big role for a small player
Exosomes uncovered. Recent research and the role of exosomes in disease
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V5-Tag: Properties
V5-Tag: Properties, origin, specifications, antibodies & Nanobodies
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Which beads should I use for my immunoprecipitation?
In detail, all beads have very low background binding due to the highly specific binding of the Nano-Traps.
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What do you get when you buy a GMP-grade product?
GMP Manufacturing, benefits, and ISO certifications explained
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Comparing BCA, Bradford, and UV protein quantification methods for scientists
Understanding the pros and cons of BCA, Bradford, and UV methods of protein quantification
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Interview with an early-career scientist: Dr Nadav Weinstock
Hear from the lead author of a landmark paper in Neuron about his research into Krabbe Disease
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How to reduce autofluorescence
Understand the causes behind autofluorescence and how to deal with this problem.
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Characterization of COVID-19 antibodies by Bio-Layer Interferometry using Nano-CaptureLigands
Binding of the immobilized COVID-19 antibodies to their target, the receptor-binding domain (RBD) of the SARS-CoV2 Spike protein, is characterized with regards to ka, kd, and KD.
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The advantages of CoraLite direct staining for IF
Video highlights of how CoraLite® antibodies can streamline your multiplex immunofluorescence staining.
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Serious about Serology: Understanding COVID19 Antibody Tests
Serious about serology: Understanding COVID19 Antibody Tests
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How fluorescent proteins can be applied in SARS-CoV-2 research
GFP or RFP and their derivatives are commonly used for virus research
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Cellular senescence and markers
How do cells come to be under "cellular arrest"? Learn what cell senescence is and how it is relevant in disease.
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SNAP-tag and CLIP-tag overview
SNAP-tag and CLIP-tag are self-labeling protein tags. The SNAP-tag protein and the CLIP-tag protein can be fused to a protein of interest (POI) and used for cellular and biochemical analysis.
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Halotag
HaloTag is a protein tag. The HaloTag protein can be fused to your protein of interest (POI) which enables it for cellular and biochemical analysis. It can also be used for live cell imaging.
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Neutralizing antibodies and their role in COVID-19 research
Weathering the Cytokine Storm
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Do you purify a special protein?
Why Spot-Tag can be used to purify any protein
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Spot-Trap or Spot-Cap?
Our Sales Rep answers one of the most frequent customer questions
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Green fluorescent protein (GFP) in plant research
Why does GFP work so well in plants?
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Virus research using GFP
References from the last 4 years in virus research using GFP and ChromoTek's GFP-Trap
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Antibody Specificity and affinity in Immunoprecipitation
What is antibody specificity and affinity? And why is it important for immunoprecipitation?
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Immunoprecipitation without additional bands
Pull-down of proteins with Nano-Traps
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GFP and RFP-Booster for better immunofluorescence imaging
ChromoTek's GFP Nanobodies and RFP Nanobodies conjugated to fluorescent dyes
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The importance of blocking when using Nano-Secondary reagents for IF
Blocking improves the sensitivity by reducing nonspecific background and therefore increases microscopy image quality. Insufficient blocking results in higher background noise.
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How to overcome the limitations of antibody-based affinity resins for protein purification
Spot-Cap for highly effective one-step protein purification
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How fellowships can advance your scientific career
Thoughts and advice to anyone thinking of applying for fellowships worldwide
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Tags for protein purification
Overview over commonly used affinity tags in protein purification
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Split fluorescent protein technology
The ChromoTek GFP-Trap has been successfully applied to multiple assays using different split GFP variants
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How to solve the genome DNA damage
DNA damage repair mechanisms: in the prevention of age-related diseases and tumorigenesis
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The best anti-GFP antibody for immunoprecipitation: GFP-Trap
Comparison of GFP-Trap and anti-GFP IgG antibody
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13 tips how to be more eco-friendly in the lab
Eco-friendly lab - Why Bother?
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Spot Capture and Detection Peptide Tag and Nanobody
The Spot Nanobody for multiple capture & detection applications of Spot-tagged proteins
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Dying to see what’s inside: Staining organelles
Visualizing the interior of organelles helps us to understand their molecular mechanisms and study cell abnormalities.
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Proteases for Western Blotting: Choosing the right tools for the job
How proteases can help you to better understand your Western blot results
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What is one-step immunostaining?
This method reduces incubation time, hands-on time and the number of washing steps.
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Tips and tricks for successful Mass spec experiments
Troubleshooting guide to address the most common obstacles during mass spectrometry experiments.
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Important facts to know when using Nano-Secondary reagents
What I should know when I'm working with Secondary antibodies or Nano-Secondary reagents
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What is the difference between necrosis and apoptosis?
Understand the differences between regulated and unregulated cell death.
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Fluorescent protein tags
Types of fluorescent protein tags and comparisons
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Missing pieces: DNA damage
Various environmental and endogenous factors cause DNA damage, resulting in cell degeneration, aging, or tumorigenesis.
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IF imaging: Widefield versus confocal microscopy
An overview of microscopy techniques and advice on when to use each method.
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Chromatin Immunoprecipitation (ChIP) with RFP-tagged or mCherry-tagged proteins
RFP-Trap for ChIP analysis in order to detect enrichment of mCherry-tagged proteins at DNA binding sites
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Mass spec essentials
An overview of mass spec sample preparation, sample purification and data analysis.
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The state of the international scientist in the U.S.
Supporting foreign-born scientists contributing to biomedical research in the U.S.
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Why studying abroad makes sense: Scientists share their story
Perspectives on Emigrating for Academic Career Advancement
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CRISPR-Cas9, TALENs and ZFNs - the battle in gene editing
Technological advances are crucial for innovative biological research. ZFNs, TALENs, and CRISPR/CRISPR-associated Cas have revolutionized genome editing.
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Quantitative MiRNA Profiling: How To Choose The Best RNA Sequencing Method?
RNA sequencing (RNA-seq) is a widely used method employing next generation sequencing (NGS) to discover and quantify the abundance of RNA molecules in biological samples.
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Are Mesenchymal Stem Cells (MSCs) true stem cells?
A guide to MSC characteristics, differentiation pathways and supporting reagents for MSC culture.
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7 secrets to a successful scientific presentation
Fight the fear of presenting your scientific research.
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A closer look at Embryonic Stem Cells (ESCs) – biological concepts
An overview of the history, features and applications of ESCs.
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TurboGFP: Properties, Sequence, MW, origin
Everything you need to know about TurboGFP: properties and applications
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Life Scientist Appreciation Week University of Chicago
June 17-21 2019. #CelebrateLifeScientists #LSAW
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Why Human Cell Expressed Proteins Are Superior for Clinical Applications
Learn about the advantages of using human cell expressed recombinant proteins in your clinical applications.
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Cell fate commitment and the Waddington landscape model
Waddington’s epigenetic landscape has been used for decades to illustrate the progressive restriction of cell differentiation during development.
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How do I know if the antibody will cross-react?
Cross-reactivity occurs when an antibody raised against one specific antigen recognizes two antigens that have similar structural regions. In this blog, we draw on our experience as antibody experts to explain why this might happen, and provide you with the tools to determine cross-reactivity in your own experiments.
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5 Tips for immunostaining using multiple labels
Our advice on microscope set-up, fluorophore choice and controls to improve your multi-label IF.
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How does blood coagulate?
Blood is an essential fluid that carries out the critical functions of transporting oxygen and nutrients to our cells.
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ICF syndrome: A gene-silencing chromatin disorder
Immunodeficiency, Centromere region instability, Facial anomalies syndrome (ICF) is a rare autosomal recessive disease that damages the immune system.
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Induced pluripotent stem cells (iPSCs) and modeling neurodegenerative disease in the dish
iPSCs and their potential for resetting the biological clock.
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Polyclonal vs. monoclonal antibodies
Both types have their unique advantages and disadvantages and can be used in a wide variety of applications.
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Epigenetic implications in brain functions - Neuroepigenetics
Environmental factors, stress, learning or drug exposure lead to active regulation of DNA structure in the nervous system.
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Proteintech supports young scientist to attend EMBL conference
Proteintech supports young scientists – Non-Coding Genome EMBL conference Heidelberg, Germany, 13–16 Sep 2017
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Actin Chromobody: Visualization of actin dynamics – NOT alteration
The ChromoTek Actin Chromobody is the only probe that does not interfere with actin filament dynamics.
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Rett syndrome - the second leading cause of mental retardation in women
An overview of Rett syndrome: clinical features, neuropathology and animal models.
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How to Write a Good Scientific Abstract
Our top tips and how to write an effective scientific abstract.
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How to be a great lab manager: 6 tips
Our advice to help you be a great lab manager and manage your time better.
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Protein or peptide antigen? Advantages and disadvantages
Peptide versus whole protein immunogens for antibody production
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Immunotherapy: boosting the immune system to fight rare cancers
Immunotherapy: how does it work and what do we know so far?
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Top Tips for a Successful Transition to a Postdoc Role
Advice and tips given by postdocs from all over the world.
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The Complexity of Proteins
How do proteins have so much functional diversity based upon only 20 building blocks?
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WNT Pathway - Defining the Canon
How did scientists figure out such a complicated pathway?
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Type 2 Diabetes Pathogenesis
Does Insulin Resistance Cause hyperinsulinemia?
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Metabolic Regulation of Cell Death
A matter of life and (cell) death...
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Epigenetics Modifications - DNA methylation
DNA Methylation: An Overview
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Alzheimer's Disease Signaling Pathway
Download or request a pathway poster for your lab
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MAPK Pathway: New Poster Available
Download or request a pathway poster for your lab
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Epigenetics - DNA layers and chromatin modeling
Introduction to Epigenetics
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mNeonGreen vs GFP
mNeonGreen: A green fluorescent protein that is not a GFP variant
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Post Translational Modifications: An Overview
Nature’s escape from genetic imprisonment
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New anti-mNeonGreen antibody & Nano-Trap for Immunofluorescence & Immunoprecipitation
Novel tools for the analysis of mNeonGreen fusion proteins
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Affinity purification, depletion, and immunoprecipitation of GST-tagged proteins
Publications using ChromoTek's GST-Trap in various applications
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Pathway Poster Library
View our interactive signaling pathways or requested a printed poster to decorate your lab
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Caspase Cascade Pathway: New Poster Available
Download a pathway poster for your lab
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The Incubator Podcast Highlights
Season 2 is here! Here's a re-cap of the topics discussed in Season 1
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Cancer stem cells as a key to cure cancer
A guide to cancer stem cells and the markers that can be used to isolate cell populations.
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How To Optimize Your ELISA Experiments
Technical tips for better ELISA results
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Immunoprecipitation of Myc-tagged proteins – How it works
ChromoTek Myc-Trap® is an optimized tool for immunoprecipitation (IP/Co-IP)
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Autophagy in the Pathogenesis of Disease
Aging, cancer, inflammation, infection, the microenvironment, and neurodegenerative disease
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9 Tips to optimize your IF experiments
Want to improve your IF experiments? Our 9 tech tips will help you achieve successful Immunofluorescence staining
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The most frequently cited monoclonal anti-RFP antibody
Immunofluorescence and immunohistochemistry with the ChromoTek's anti-RFP antibody [5F8]
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Atrial and ventricular myocytes
A guide to atrial and ventricular myocyte markers
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A beginners guide to Immunohistochemistry
12 steps to get you started with immunohistochemistry
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Lysate Preparation: How Do I Optimize My Extraction?
Technical tips to improve your sample preparation for Western blot
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Lysate Preparation: Why is RIPA Buffer Best for Western Blot
What is in a lysis buffer? Learn the purpose of each component.
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Visualize the onset of metastasis with Chromobodies
The potential of the chromobody technology to study EMT in cell-based screening systems is reviewed by Maier et al., Scientific Reports 2015.
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Mitochondria: More than just the powerhouse of the cell
An introduction to mitochondria in health and disease.
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8 Top Tips For Immunoprecipitation
8 tips on how to perform better IP experiments.
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Hidden Secrets Of The Heart
The plot thickens… and so does the artery wall!
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Mitochondria: Center of Cellular Signaling and Energetic Balance
Mitochondrial markers
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Career Development Podcast: The Incubator
Fret over your scientific career no longer. Proteintech is sponsoring a monthly career development podcast, The Incubator.
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10 tips on how to best optimize siRNA transfection
Improve your siRNA transfections with these expert tips.
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The Neurochemistry of Parkinson's Disease
An overview of brain neurochemistry in Parkinson's Disease, written by guest blogger Ashley Juavinett.
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Molecular markers for liver cancer
Discover molecular markers for hepatocellular carcinoma.
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Nano-Booster FAQs
Frequently asked technical questions on GFP-and RFP-Booster
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How to elute GFP-fusion protein from the GFP-Trap®
Tips and tricks
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Loading Control Antibodies for Western Blotting
Discover the most appropriate loading controls for your Western blot experiments.
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Nano Diamonds and Cancer
An introduction to nanoparticle delivery in cancer, written by guest blogger Veronika Benson.
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Fast Phospho-rward: Making Phosphoprotein Antibodies
A historical perspective on the production of phospo-antibodies.
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Immunofluorescence: Tips for Immunostaining Cultured Cells
Improve your IF with these expert tips from Proteintech scientists.
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Recalling when and where: How the brain processes episodic memory
Introduction
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8 Top Tips for Subcellular Fractionation
8 technical tips for successful subcellular fractionation
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Antibodies for Cilia Development
A guide to key targets in cilia development and ciliogenesis.
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Tech Tips | In search of low molecular weight proteins
Learn how to improve your Western blot for low molecular weight proteins.
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Can a Bacterial 'Brain' Control a Robot?
A perspective on how technology can be used to control biological systems, by guest blogger Ashley Juavinett
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Making the connection between brain and body
We’re connected, from head to toe...
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GSK3 pathway poster
Request a printed copy of the GSK signaling pathway for your lab.
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New nuclear Actin-Chromobody® plasmid
It is our pleasure to announce the availability of a new member of our Actin Chromobody® plasmid family:
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Can man's best friend help cure cancer
Humans are not the only species on earth that develop cancer; our beloved pets can also succumb to the ‘Big C’ too.
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Seeing Clearly: Tips For Staining Cleared Tissue With Antibodies
Improve your immunofluorescence in cleared tissue with expert tips from our guest blogger, Ashley Juavinett.
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I Just Want Your Extra Time And Your...KISS-1
Learn about the function of the KISS-1 protein, written by guest blogger Sophie Quick.
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Breast Cancer | 7 Interesting Facts
Learn something you didn't know about breast cancer.
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MHC Class I: The Cancer Connection
The relationship between the major histocompatibility complex (MHC) and cancer.
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7 Tips For Optimizing Your Western Blotting Experiments
How to optimize your western blot protocol
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Special offer for newsletter sign up
Sign up to the Proteintech regular eNewsletter to receive a free lab survival kit.
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Antibody Comparison Sites: How to Find Your Perfect Antibody Faster
A guide to finding the best antibody for your application
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Example PhD interview questions and answers
How to succeed in your PhD interview with answers to frequently asked questions
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The multi-organ impact of COVID-19
Written by Alicia Haydo M.Sc., a PhD candidate at Goethe University Frankfurt am Main
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COVID-19 Neuropathology Similar to Alzheimer’s Disease – The Role of the Ryanodine Receptor in “Long COVID”
Written by Leah Sittenfeld, M.D. Candidate at New York Medical College
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Second Cancer After Breast Cancer
Explore why breast cancer survivors are more likely to develop a second cancer after the first one has been cured.
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Primary Cilia and Regulation of Blood Pressure (“The Silent Killer”)
Discovering cilia’s role as a regulator of arterial blood vessels and its involvement in disease.
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Imaging the Rainbow: Multiplexing with Same-Species Antibodies for Immunofluorescence
Improve your IF multiplexing with these expert tips from our guest blogger, Derek Sung (IG: @Immunofluoresence)
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How to succeed in an interview – Academia vs. Industry
Find out how to best prepare for an interview with top tips from our experts with successful academic and industry careers.
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How to write an industry resume/CV
Are you a life-scientist looking to change your career path? Learn how to write an industry resume/CV
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Ask the Experts: Transitioning from Academia to Industry
Read some of the engaging Q&A session from our recent careers workshop on moving from an academic to industry position.
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JAK/STAT Signaling: New Pathway Poster
Download a pathway poster for your lab
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